Recombinant Human CTP synthase 1 (CTPS1)

中文名稱：

Recombinant Human CTP synthase 1(CTPS1)
貨號(hào)：

CSB-YP006176HU
說明書：

MSDS Datasheet
規(guī)格：

￥1500
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
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靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

CTPS1
Uniprot No.：

P17812
別名：

CTPS1; CTPS; CTP synthase 1; EC 6.3.4.2; CTP synthetase 1; UTP--ammonia ligase 1
種屬：

Homo sapiens (Human)
蛋白長(zhǎng)度：

Full Length
來源：

Yeast
分子量：

68.7kDa
表達(dá)區(qū)域：

1-591aa
氨基酸序列

MKYILVTGGVISGIGKGIIASSVGTILKSCGLHVTSIKIDPYINIDAGTFSPYEHGEVFVLDDGGEVDLDLGNYERFLDIRLTKDNNLTTGKIYQYVINKERKGDYLGKTVQVVPHITDAIQEWVMRQALIPVDEDGLEPQVCVIELGGTVGDIESMPFIEAFRQFQFKVKRENFCNIHVSLVPQPSSTGEQKTKPTQNSVRELRGLGLSPDLVVCRCSNPLDTSVKEKISMFCHVEPEQVICVHDVSSIYRVPLLLEEQGVVDYFLRRLDLPIERQPRKMLMKWKEMADRYDRLLETCSIALVGKYTKFSDSYASVIKALEHSALAINHKLEIKYIDSADLEPITSQEEPVRYHEAWQKLCSAHGVLVPGGFGVRGTEGKIQAIAWARNQKKPFLGVCLGMQLAVVEFSRNVLGWQDANSTEFDPTTSHPVVVDMPEHNPGQMGGTMRLGKRRTLFQTKNSVMRKLYGDADYLEERHRHRFEVNPVWKKCLEEQGLKFVGQDVEGERMEIVELEDHPFFVGVQYHPEFLSRPIKPSPPYFGLLLASVGRLSHYLQKGCRLSPRDTYSDRSGSSSPDSEITELKFPSINHD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

The intact gene sequence encoding the human CTP synthase 1 (CTPS1) is expressed in the yeast. The resulting protein is fused with the 6xHis-tag to generate the recombinant human CTPS1 protein. Its purity reaches up to 90% measured by SDS-PAGE. On the gel, this recombinant CTPS1 protein migrated to the molecular weight band of about 70 kDa. The observed molecular weight is a little greater than the theoretical value due to the galactosylated modification. The target protein CTPS1 catalyzes the rate-limiting step in the de novo synthesis of CTP. It is important for the expansion of activated T cells. In T lymphocytes, CTPS1 expression is upregulated in response to TCR stimulation.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

問答及客戶評(píng)論

■ 常見問題解答

Can you help me out with the following questions:
1. The above has been purified by his tag and then dialysed? Or a different way?
2. Have you tested (or could you test activity)

Thanks for your inquiry. The protein information is as follow:
1）The tag of this protein is N-terminal 6xHis-tagged, and we purify through his-tag.
2）We haven't tested the activity of this protein yet so we can't 100% guarantee that it is active. The protein is purified under mild conditions, which should have activity in theory. Here is a link for reference. https://europepmc.org/articles/PMC1400552;jsessionid=EF3A6F1F8903A542A07957B8594C1A8A
It shows phosphorylation is more important for this protein, and yeast expression protein is active. You can refer to it.

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靶點(diǎn)詳情

功能：

This enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity.
基因功能參考文獻(xiàn)：
1. CTP synthase forms the cytoophidium in human hepatocellular carcinoma. PMID: 29097181
2. The filament structures elucidate allosteric mechanisms of assembly and regulation that rely on a conserved conformational equilibrium. The findings may provide a mechanism for increasing human CTP synthase activity in response to metabolic state and challenge the assumption that metabolic filaments are generally storage forms of inactive enzymes. PMID: 28459447
3. Rods and rings (RR) are protein assemblies composed of CTPS1 and IMPDH2. Glutamine deprivation initiates reversible assembly of mammalian rods and rings. PMID: 24477477
4. results highlight a key and specific role of CTPS1 in the immune system by its capacity to sustain the proliferation of activated lymphocytes during the immune response PMID: 24870241
5. analysis of the kinetic properties of hCTPS1 and hCTPS2, and determination that Ser(68) is a major site of CTPS2 regulation by phosphorylation PMID: 20739275
6. CTP synthetase is phosphorylated by protein kinase A PMID: 16179339
7. Data indicate that protein kinase C phosphorylation at Ser(462) stimulates human CTP synthetase 1 activity, whereas phosphorylation at Thr(455) inhibits activity. PMID: 17463002
8. analysis of phosphorylation and regulation of human CTPS1 in human cells shows that GSK3 is a novel regulator of CTPS activity PMID: 17681942
9. studies provide novel information on the potential interacting proteins that may regulate CTPS1 function or intracellular localization PMID: 18600551
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相關(guān)疾病：

Immunodeficiency 24 (IMD24)
亞細(xì)胞定位：

Cytoplasm, cytosol.
蛋白家族：

CTP synthase family
組織特異性：

Widely expressed.
數(shù)據(jù)庫鏈接：

HGNC: 2519

OMIM: 123860

KEGG: hsa:1503

STRING: 9606.ENSP00000361699

UniGene: Hs.473087