多克隆抗體定制服務(wù)
多克隆抗體存在于免疫動(dòng)物的血清中，可通過直接分離血清獲得。由于具有可識(shí)別多個(gè)表位、可引起凝集反應(yīng)、沉淀反應(yīng)等優(yōu)點(diǎn)，被廣泛應(yīng)用于免疫學(xué)診斷領(lǐng)域。比如經(jīng)常用到的Western blotting實(shí)驗(yàn)中的酶標(biāo)二抗和ELISA實(shí)驗(yàn)中的包被抗體。而且制備費(fèi)用也較單克隆抗體低。
華美生物自主建立抗體研發(fā)生產(chǎn)平臺(tái)，可為您提供可靠的多克隆抗體定制服務(wù)，并有多種免疫原和多種宿主物種可供選擇。您可以選擇自己提供抗原或者我們幫您設(shè)計(jì)并制備抗原，我們將提供給您滿足您實(shí)驗(yàn)需求的多克隆抗體產(chǎn)品。
服務(wù)流程：
服務(wù)內(nèi)容及周期：
| 免疫原 | 服務(wù)內(nèi)容 | 交付內(nèi)容 | 服務(wù)周期 |
|---|---|---|---|
| 多肽 |
|
|
12-14 周 |
| 重組蛋白 | 14-16 周 | ||
| 天然蛋白 | 12-14 周 |
服務(wù)實(shí)例：
ELISA
Antigen coating concentration 2 ug/ml
Antiserum 1:2000 is more than diluted
Secondary：Goat polyclonal to rabbit IgG at 1/50000 dilution
Western Blot
Positive WB detected in：K562 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate,Rat liver tissue, Mouse brain tissue, Mouse lung tissue
All lanes：a antibody at 2ug/ ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64 KDa
Observed band size: 64 KDa
Western Blot
WT: Wild-type 293 cells
KO: Knockout 293 cells
IHC image of A antibody diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with A antibody at 1:400,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
聯(lián)系信息
電話：027-87002556
Q Q：1031906505 
地址：武漢市東湖新技術(shù)開發(fā)區(qū)高新大道818號(hào)高科醫(yī)療器械園B11號(hào)2樓
華美生物︱YOUR GOOD PARTNER IN BIOLOGY RESEARCH!
武漢華美生物工程有限公司成立于2007年12月，是一家集科研、生產(chǎn)、銷售為一體的生物高新技術(shù)企業(yè)。并形成了以CUSABIO為品牌的ELISA試劑盒、蛋白、抗體等多種優(yōu)質(zhì)產(chǎn)品，遠(yuǎn)銷歐美日德等九十多個(gè)國(guó)家。
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