THEMIS Recombinant Monoclonal Antibody
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中文名稱：THEMIS重組抗體
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貨號：CSB-RA262013A0HU
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規(guī)格：￥1320
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圖片：
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IHC image of CSB-RA262013A0HU diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.30% DAB. -
Immunofluorescence staining of HepG2 with CSB-RA262013A0HU at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 503-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing Jurkat cells stained with CSB-RA262013A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA262013A0HU THEMIS重組單克隆抗體是經(jīng)多種實驗驗證的高特異性科研工具，適用于ELISA、免疫組化（IHC）、免疫熒光（IF）及流式細胞術(shù)（FC）檢測，推薦工作濃度為1:50-1:200。該抗體針對THEMIS蛋白設(shè)計，該靶標作為T細胞受體信號通路的關(guān)鍵調(diào)控因子，在胸腺細胞發(fā)育、T細胞活化及免疫耐受建立中發(fā)揮重要作用。經(jīng)嚴格驗證，本產(chǎn)品在石蠟包埋組織切片中能清晰顯示細胞質(zhì)定位信號，在免疫熒光實驗中呈現(xiàn)穩(wěn)定的亞細胞染色特異性，流式檢測可有效識別細胞表面抗原表達，所有實驗均表現(xiàn)出優(yōu)異的批間一致性和低背景干擾。適用于研究T細胞分化機制、免疫調(diào)控網(wǎng)絡(luò)及自身免疫性疾病模型構(gòu)建，可配合多種樣本類型（如冰凍/石蠟組織、原代細胞或培養(yǎng)細胞系）進行定量或定性分析，為免疫學(xué)基礎(chǔ)研究及腫瘤微環(huán)境相關(guān)課題提供可靠檢測方案。
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Uniprot No.：
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基因名：THEMIS
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別名：Protein THEMIS (Thymocyte-expressed molecule involved in selection), THEMIS, C6orf190 C6orf207
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide derived from Human THEMIS
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：7G7
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
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基因功能參考文獻：
- we have demonstrated mutually exclusive use of exon 1 and 2 amongst 16 novel THEMIS isoforms. We also show inverse correlation between THEMIS expression in human CD4+ T-cells and dosage of the multiple sclerosis risk allele at rs13204742, driven by reduced expression of exon 1- containing isoforms PMID: 27438997
- we found that THEMIS directly regulated the catalytic activity of the tyrosine phosphatase SHP-1. PMID: 28250424
- the THEMIS:SHP complex dampens early TCR signalling by a previously unknown molecular mechanism that favours T-cell survival. PMID: 25535246
- THEMIS showed higher mRNA expression in active celiac disease compared with treated patients and controls. PMID: 23820479
- THEMIS binding to GRB2 was required for thymocyte development. PMID: 23460737
- Themis1 exhibits the same potential to restore T cell development and T cell receptor-mediated signaling in Themis1-deficient mice. PMID: 22732588
- SPOP mutations may define a new molecular subtype of prostate cancer. PMID: 22610119
- specific role for THEMIS in early TCR signalosome function PMID: 21189249
- Observational study of gene-disease association. (HuGE Navigator) PMID: 19913121
- Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
- Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20095854
- Functional characterization of the homologous mouse gene. PMID: 19597498
- Functional analysis of the mouse gene by knockout, and comparison of the mouse and human proteins. PMID: 19597499
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亞細胞定位：Cytoplasm. Nucleus.
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蛋白家族：Themis family
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數(shù)據(jù)庫鏈接：
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