THEMIS Antibody

中文名稱：

THEMIS兔多克隆抗體
貨號(hào)：

CSB-PA850780LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate
All lanes: THEMIS antibody at 7.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 74, 70, 63, 78 kDa
Observed band size: 74, 70 kDa

IHC image of CSB-PA850780LA01HU diluted at 1:300 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA850780LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) THEMIS Polyclonal antibody
Uniprot No.：

Q8N1K5
基因名：

THEMIS
別名：

C6orf190 antibody; C6orf207 antibody; MGC163388 antibody; Protein THEMIS antibody; SIGNALING PHOSPHOPROTEIN SPECIFIC FOR T CELLS antibody; SPOT antibody; THEMIS antibody; THMS1_HUMAN antibody; Thymocyte expressed molecule involved in selection antibody; Thymocyte selection associated antibody; Thymocyte selection pathway associated antibody; Thymocyte-expressed molecule involved in selection antibody; TSEPA antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Protein THEMIS protein (547-641AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，THEMIS Antibody (CSB-PA850780LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于THEMIS Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA850780LB01HU	THEMIS Antibody, HRP conjugated	ELISA
FITC	CSB-PA850780LC01HU	THEMIS Antibody, FITC conjugated
Biotin	CSB-PA850780LD01HU	THEMIS Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
基因功能參考文獻(xiàn)：
1. we have demonstrated mutually exclusive use of exon 1 and 2 amongst 16 novel THEMIS isoforms. We also show inverse correlation between THEMIS expression in human CD4+ T-cells and dosage of the multiple sclerosis risk allele at rs13204742, driven by reduced expression of exon 1- containing isoforms PMID: 27438997
2. we found that THEMIS directly regulated the catalytic activity of the tyrosine phosphatase SHP-1. PMID: 28250424
3. the THEMIS:SHP complex dampens early TCR signalling by a previously unknown molecular mechanism that favours T-cell survival. PMID: 25535246
4. THEMIS showed higher mRNA expression in active celiac disease compared with treated patients and controls. PMID: 23820479
5. THEMIS binding to GRB2 was required for thymocyte development. PMID: 23460737
6. Themis1 exhibits the same potential to restore T cell development and T cell receptor-mediated signaling in Themis1-deficient mice. PMID: 22732588
7. SPOP mutations may define a new molecular subtype of prostate cancer. PMID: 22610119
8. specific role for THEMIS in early TCR signalosome function PMID: 21189249
9. Observational study of gene-disease association. (HuGE Navigator) PMID: 19913121
10. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
11. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20095854
12. Functional characterization of the homologous mouse gene. PMID: 19597498
13. Functional analysis of the mouse gene by knockout, and comparison of the mouse and human proteins. PMID: 19597499
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亞細(xì)胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Themis family
數(shù)據(jù)庫鏈接：

HGNC: 21569

OMIM: 613607

KEGG: hsa:387357

UniGene: Hs.661756

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