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中文名稱：大鼠1,4,5-三磷酸肌醇(IP3)酶聯(lián)免疫試劑盒
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貨號：CSB-E13004r
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規(guī)格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：大鼠1,4,5-三磷酸肌醇(IP3)酶聯(lián)免疫試劑盒 （CSB-E13004r）為競爭法ELISA試劑盒，定量檢測血清、血漿、tissue 勻漿、組織培養(yǎng)上清液、細胞裂解物樣本中的IP3含量。IP3（肌醇三磷酸）是一種重要的細胞信號分子，由磷脂酶C（PLC）水解磷脂酰肌醇-4,5-二磷酸（PIP2）產生。它作為第二信使，通過結合內質網上的IP3受體，觸發(fā)鈣離子釋放，從而調節(jié)細胞內鈣信號，影響多種細胞反應，如平滑肌收縮、血小板聚集等。研究IP3信號轉導機制有助于理解相關疾病的發(fā)生和發(fā)展，為疾病治療提供新靶點。試劑盒檢測范圍為5 pg/mL-1000 pg/mL，該產品適用于神經生物學、心血管疾病模型、藥物作用機制等研究領域，可用于探索細胞內信號通路、評估化合物干預效果。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
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縮寫：IP3
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates, cell culture supernates, cell lysates
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檢測范圍：5 pg/mL-1000 pg/mL
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靈敏度：2.5 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Signal Transduction
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測定原理：quantitative
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測定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 85-96 1:2 Average % 94 Range % 89-98 1:4 Average % 96 Range % 87-102 1:8 Average % 97 Range % 91-104 -
回收率：
The recovery of rat IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 91-105 EDTA plasma (n=4) 95 92-98 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 1000 0.141 0.143 0.142 250 0.270 0.261 0.266 80 0.423 0.440 0.432 20 0.744 0.759 0.752 5 1.156 1.162 1.159 0 2.199 2.214 2.207 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻
- Selective Inhibition of P2Y1 and P2Y12 Receptor Signal Pathways in Platelet Aggregation in Transgenic Cell Lines and Rats by Potassium 2-(1-Hydroxypentyl)-Benzoate, Puerarin and Salvianolic Acid B Y Li, W Wang, J Cai, N Feng, S Xu,CNS Neuroscience & Therapeutics,2024
- Anandamide modulates WNT-5A/BCL-2, IP3/NFATc1, and HMGB1/NF-κB trajectories to protect against mercuric chloride-induced acute kidney injury DM Abdallah,Scientific reports,2023
- Nitric Oxide Linked to mGluR5 Upregulates BDNF Synthesis by Activating MMP2 in the Caudate and Putamen after Challenge Exposure to Nicotine in Rats J Kim,International journal of molecular sciences,2022
- Kaempferol suppresses acetaminophen-induced liver damage by upregulation/activation of SIRT1 Nora Abdullah AlFaris,Pharmaceutical Biology,2021
- Amphetamine-induced conditioned place preference and changes in mGlu1/5 receptor expression and signaling in the rat medial prefrontal cortex Mao LM, et al,Neuroscience,2018
- Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling Ji Eun Kim, et al,International Journal of Molecular Medicine,2018
- Molecular mechanism of Danshensu on platelet antiaggregation Chen Yu.et al,American Institute of Physics,2018
- Alterations in mGlu5 receptor expression and function in the striatum in a rat depression model Mao LM.et al,Journal of Neurochemistry,2018
- Melatonin Promotes Brain-Derived Neurotrophic Factor (BDNF) Expression and Anti-Apoptotic Effects in Neonatal Hemolytic Hyperbilirubinemia via a Phospholipase Yong Luo.et al,Medical Science Monitor 23,2017
- Protective role of Pollen Typhae total flavone against the palmitic acid-induced impairment of glucose-stimulated insulin secretion involving GPR40 signaling in INS-1 cells Feng XT.et al,Int J Mol Med,2017
- Uridine stimulate laxative effect in the loperamide-induced constipation of SD rats through regulation of the mAChRs signaling pathway and mucin secretion Ji Eun Kim,.et al,BMC Genet.,2017
- Effects of five candidate laxatives derived from Liriope platyphylla on the 5-HT receptor signaling pathway in three cell types present in the transverse colon Ji Eun Kim.et al,Molecular Medicine Reports,2016
- A long-acting β2-adrenergic agonist increases the expression of muscarine cholinergic subtype Liu YH.et al,Mol Med Rep.,2015
- A long-acting ß2-adrenergic agonist increases the expression of muscarine cholinergic subtype-3 receptors by activating the ß2-adrenoceptor cyclic adenosine monophosphate signaling pathway in airway smooth muscle cells. Yuan-Hua Liu et al,Molecular Medicine reports,2015
- Lipid peroxidation is essential for phospholipase C activity and IP3 related calcium signal Domijan AM et al,J Cell Sci,2013
- Effect of FSH receptor-binding inhibitor-8 on FSH-mediated granulosa cell signaling and proliferation. Navalakhe RM et al,Chem Biol Drug Des,2013
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