Mouse inositol 1,4,5,-trisphosphate(IP3) ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠1,4,5-三磷酸肌醇(IP3)酶聯免疫試劑盒（CSB-E13410m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿、細胞裂解物樣本中的IP3含量。IP3（肌醇三磷酸）是一種重要的細胞信號分子，參與G蛋白偶聯受體介導的信號轉導。它由PLC水解PIP2產生，能結合內質網上的IP3受體，引發鈣離子釋放，進而調控細胞內多種生理過程。研究IP3信號通路有助于理解細胞內鈣信號調控機制及其在疾病發生中的作用。試劑盒檢測范圍為0.78 pg/mL-50 pg/mL，適用于實驗室研究細胞信號通路激活、受體功能評估或藥物干預后IP3動態變化。為探索IP3在生理病理機制中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IP3
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
檢測范圍：

0.78 pg/mL-50 pg/mL
靈敏度：

0.195 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	82-95
1:200	Average %	95
	Range %	91-99
1:400	Average %	97
	Range %	96-105
1:800	Average %	98
	Range %	89-102

回收率：

The recovery of mouse IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-107
EDTA plasma (n=4)	98	92-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.