Human inositol 1,4,5,-trisphosphate(IP3) ELISA kit

Human inositol 1,4,5,-trisphosphate(IP3) ELISA kit

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中文名稱：

人1,4,5-三磷酸肌醇(IP3)酶聯免疫試劑盒
貨號：

CSB-E12636h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人1,4,5-三磷酸肌醇(IP3)酶聯免疫試劑盒（CSB-E12636h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IP3含量。IP3即三磷酸肌醇，是細胞內重要的第二信使。其背景在于它在細胞信號轉導中扮演關鍵角色。研究機制上，當細胞外信號激活相應受體，通過G蛋白激活磷脂酶C，水解磷脂酰肌醇生成IP3，它能促使內質網釋放Ca2?，引發一系列細胞反應。試劑盒檢測范圍為5 pg/mL-1000 pg/mL，尤其適用于體外研究細胞信號通路激活程度、藥物靶點作用機制或疾病模型中IP3動態變化的分析；為探究G蛋白偶聯受體介導的磷酸肌醇代謝、細胞應激反應調控等基礎研究提供可靠工具，廣泛適配于神經生物學、藥理學及病理生理學等領域的科研需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IP3
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

5 pg/mL-1000 pg/mL
靈敏度：

2.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-105
1:2	Average %	94
	Range %	89-100
1:4	Average %	94
	Range %	87-102
1:8	Average %	95
	Range %	90-102

回收率：

The recovery of human IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-100
EDTA plasma (n=4)	92	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.202	0.211	0.207
250	0.350	0.349	0.350
80	0.600	0.594	0.597
20	1.029	1.018	1.024
5	1.326	1.345	1.336
0	2.102	2.130	2.116

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Second messenger role for Mg21 revealed by human T-cell immunodeficiency Feng-Yen Li,Nature,2011
C6-ceramide induces salivary adenoid cystic carcinoma cell apoptosis via IP3R-activated UPR and UPR-independent pathways L Qiu,Biochem. Biophys. Res. Commun,2020
High-dose levocetirizine for the treatment of refractory chronic spontaneous urticaria and the effect on the serum inositol triphosphate level Huang X, et al,Journal of International Medical Research,2019
Activation of calcium signaling in human gingival fibroblasts by recombinant Porphyromonas gingivalis RgpB protein Li S,european journal of oral sciences,2019
Inositol 1,4,5-trisphosphate (IP3)-dependent Ca2+ signaling mediates delayed myogenesis in Duchenne muscular dystrophy fetal muscle Farini A.et al,Development.,2016
Combined assessment of polymorphisms in the LHCGR and FSHR genes predict chance of pregnancy after in vitro fertilization Lindgren I. et al,Hum Reprod,2016
Cilostazol Induces PGI 2 Production via Activation of the Downstream Epac-1/Rap1 Signaling Cascade to Increase Intracellular Calcium by PLC(Epsilon) and to Activate p44/42 MAPK in Human Aortic Endothelial Cells. Hashimoto A et al,PLoS One,2015
Octopamine Regulates Antennal Sensory Neurons via Daytime-Dependent Changes in cAMP and IP3 Levels in the Hawkmoth Manduca sexta. Schendzielorz T et al,PLoS One,2015
Cytochrome C dysregulation induced by HIV infection of astrocytes results in bystander apoptosis of uninfected astrocytes by an IP3 and calcium-dependent mechanism Eugenin EA et al,J Neurochem,2013
Second messenger role for Mg2+ revealed by human T-cell immunodeficiency /,Nature,2011

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■ 常見問題解答

I would like to receive more information about this assay, particularly regarding specificity for IP3.
Please provide some more information about the following.
a. capture antibody or capture antigen
b. Detection antibody (if applicable)
c. standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.
Are you aware of any product specific references for this assay? If yes please can you provide links to these references. If you have any other information about validation of the antibodies in other applications e.g. WB this information would be helpful also.

Thanks for your inquiry.
CSB-E12636h
This is a competitive kit.
The plate is coated with goat-anti-rabbit antibody.
The specific antibody is rabbit polyclonal antibody.
The standard is purified IP3.
The raw materials of the kit have been screened strictly and passed quality control. Sorry it is not convenient for us to provide validation information of the antibodies because it is only for our internal tracing. There are many references about this kit. Pls find following links:
1. https://www.ncbi.nlm.nih.gov/pubmed/?term=26884398 https://academic.oup.com/humrep/article/31/3/672/2384908
3. https://www.ncbi.nlm.nih.gov/pubmed/26181635
4.https://www.ncbi.nlm.nih.gov/pubmed/25785721
5. https://www.ncbi.nlm.nih.gov/pubmed/23992092
6. https://www.nature.com/articles/nature10246?WT.ec_id=NATURE-20110728
Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.