Rat angiotensin 1-7 (Ang1-7) ELISA kit

Rat angiotensin 1-7 (Ang1-7) ELISA kit

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文獻引用 (9) 說明書下載

中文名稱：

大鼠血管緊張素1-7(Ang1-7)酶聯(lián)免疫試劑盒
貨號：

CSB-E14241r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠血管緊張素1-7(Ang1-7)酶聯(lián)免疫試劑盒（CSB-E14241r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Ang1-7含量。Ang1-7是一種血管緊張素，作為腎素-血管緊張素系統(tǒng)（RAS）的組成部分，其作用與AngⅡ相反。研究顯示，Ang1-7可通過調節(jié)Rac1、Rad等蛋白表達，抑制成纖維細胞合成細胞外基質，從而對心血管系統(tǒng)產(chǎn)生保護作用。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于基礎科研中評估心血管疾病模型、代謝紊亂或器官損傷等實驗條件下Ang1 - 7的動態(tài)變化，在高血壓、糖尿病腎病等疾病機制研究中具有重要價值，滿足實驗室對生物活性分子定量分析的需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Ang1-7
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.68 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	80-105
1:2	Average %	96
	Range %	85-104
1:4	Average %	102
	Range %	92-114
1:8	Average %	99
	Range %	80-110

回收率：

The recovery of rat Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	83-112
EDTA plasma (n=4)	95	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	3.121	3.129	3.125	2.986
600	2.532	2.522	2.527	2.388
300	1.739	1.765	1.752	1.613
150	1.050	1.041	1.046	0.907
75	0.623	0.642	0.633	0.494
37.5	0.435	0.447	0.441	0.302
18.75	0.265	0.254	0.260	0.121
0	0.135	0.143	0.139

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Chicken Muscle-Derived ACE2 Upregulating Peptide VVHPKESF Inhibits Angiotensin II-Stimulated Inflammation in Vascular Smooth Muscle Cells via the ACE2/Ang (1–7)/MasR Axis H Fan,Journal of agricultural and food chemistry,2023
Biomimetic Nanoparticle-Mediated Target Delivery of Hypoxia-Responsive Plasmid of Angiotensin-Converting Enzyme 2 to Reverse Hypoxic Pulmonary Hypertension R Yuan,ACS nano,2023
The Expression of RAAS Key Receptors, Agtr2 and Bdkrb1, Is Downregulated at an Early Stage in a Rat Model of Wolfram Syndrome M Punapart,Genes,2021
CoQ10 augments candesartan protective effect against tourniquet-induced hind limb ischemia-reperfusion: involvement of non-classical RAS and ROS pathways AS Awad,Saudi Pharmaceutical Journal,2021
Vitamin A deficiency indicating as low expression of LRAT may be a novel biomarker of primary hypertension X Liang,Journal Clinical and Experimental Hypertension,2020
The contribution of chymase-dependent formation of AngII to cardiac dysfunction in metabolic syndrome of young rats: roles of fructose and EETs G Froogh,Am J Physiol Heart Circ Physiol,2020
Dynamic Variation of RAS on Silicotic Fibrosis Pathogenesis in Rats Zhang BN, et al,Current Medical Science,2019
Protective Effect of Angiotensin (1-7) on Silicotic Fibrosis in Rats Zhang BN, et al,Biomedical and Environmental Sciences,2019
Blood pressure lowering effect of Ficus deltoidea var kunstleri in spontaneously hypertensive rats: possible involvement of renin-angiotensin-aldosterone system, endothelial function and anti-oxidant system Azis NA, et al,Molecular Biology Reports,2019

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.