Human Angiotensin 1-7(Ang1-7)ELISA Kit

產品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人血管緊張素1-7（Ang1-7）酶聯免疫試劑盒（CSB-E14242h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液樣本中的Ang1-7含量。Ang1-7是一種血管緊張素，具有血管舒張作用，影響心血管器官如心臟、血管和腎臟。研究顯示，Ang1-7通過降低ERK1/2MAPK活性，調節心血管功能，并在肺纖維化、肝癌轉移等疾病中發揮作用。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于心血管疾病機制研究、藥物干預效果評估、細胞模型中Ang1-7分泌水平檢測等科研領域，尤其適合低豐度樣本的定量分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

Ang1-7
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-105
1:2	Average %	96
	Range %	85-109
1:4	Average %	105
	Range %	92-114
1:8	Average %	95
	Range %	82-110

回收率：

The recovery of human Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-110
EDTA plasma (n=4)	99	90-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.