Mouse adrenocorticotropic hormone (ACTH) ELISA kit
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中文名稱：小鼠促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒
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貨號：CSB-E06874m
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規(guī)格：96T/48T
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價格：￥3800/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒（CSB-E06874m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的ACTH含量。ACTH即促腎上腺皮質(zhì)激素，由腺垂體分泌。其背景在于它能促進腎上腺皮質(zhì)的組織增生以及皮質(zhì)激素的生成和分泌。研究機制方面，它作用于腎上腺皮質(zhì)細(xì)胞上的受體，激活相關(guān)信號通路，調(diào)控皮質(zhì)激素合成關(guān)鍵酶的表達(dá)，進而調(diào)節(jié)機體應(yīng)激等生理過程。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，該產(chǎn)品適用于實驗動物模型中ACTH動態(tài)變化的分析，例如應(yīng)激反應(yīng)評估、腎上腺功能研究、內(nèi)分泌調(diào)控機制探索以及藥物或環(huán)境因素對激素分泌影響的科研實驗，為神經(jīng)內(nèi)分泌領(lǐng)域的定量研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：ACTH
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma
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檢測范圍：18.75 pg/mL-1200 pg/mL
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靈敏度：4.68 pg/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Metabolism
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 80-105 1:2 Average % 96 Range % 85-104 1:4 Average % 98 Range % 92-104 1:8 Average % 96 Range % 82-110 -
回收率：
The recovery of mouse ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 84-102 EDTA plasma (n=4) 98 90-105 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1200 3.134 3.126 3.130 2.988 600 2.537 2.532 2.535 2.393 300 1.754 1.765 1.760 1.618 150 1.053 1.050 1.052 0.910 75 0.651 0.647 0.649 0.507 37.5 0.443 0.435 0.439 0.297 18.75 0.273 0.265 0.269 0.127 0 0.140 0.144 0.142 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Xiao‐Chai‐Hu‐Tang Ameliorates Depressive Symptoms via Modulating Neuro‐Endocrine Network in Chronic Unpredictable Mild Stress‐Induced Mice Y Feng, W Wang, Y Zhang, Y Feng,CNS Neuroscience & Therapeutics,2025
- Prenatal p25-activated Cdk5 induces pituitary tumorigenesis through MCM2 phosphorylation-mediated cell proliferation Y Huang, Q Wang, W Zhou, Y Jiang, K He, W Huang,Neoplasia,2024
- Excessive free fatty acid sensing in pituitary lactotrophs elicits steatotic liver disease by decreasing prolactin levels X Ji,Cell reports,2024
- Single-cell transcriptomic analysis reveals rich pituitary–Immune interactions under systemic inflammation T Yan,PLoS biology,2023
- Dendrobium officinale polysaccharides alleviate depression-like symptoms via regulating gut microbiota-neuroinflammation in perimenopausal mice QP Zhang,Journal of Functional Foods,2021
- Intranasal Oxytocin Administration Ameliorates DSS-induced Abnormal Stress-related Behavior and Intestinal Inflammation in an IBD Mouse Model C Liu,Authorea Preprints,2020
- Magnolol abrogates chronic mild stress-induced depressive-like behaviors by inhibiting neuroinflammation and oxidative stress in the prefrontal cortex of mice Cheng J.et al,Int Immunopharmacol.,2018
- Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota Ren W.et al,J Pineal Res.,2017
產(chǎn)品評價
平均分:
樣品類型：血清
樣品信息：小鼠
稀釋比：其他 1:4
產(chǎn)品評價： 使用CSB-E06874m試劑盒檢測小鼠血清ACTH水平，稀釋4倍后正常與模型組及各給藥組大部分均在標(biāo)曲范圍內(nèi)，但如果樣品充足可考慮1:1稀釋或使用原液，換算稀釋倍數(shù)后原液仍在標(biāo)曲范圍內(nèi)。全部實驗在6小時內(nèi)完成，檢測結(jié)果較穩(wěn)定，標(biāo)曲良好.
By 蘭老師
樣品類型：血清
樣品信息：小鼠
稀釋比：其他 1:5
產(chǎn)品評價： 小鼠血清稀釋5倍后依然在標(biāo)曲檢測范圍內(nèi)，節(jié)省樣本，對珍貴樣本極其友好；而且復(fù)孔間差異很小，兩個復(fù)孔吸光值幾乎一致，質(zhì)量很好的試劑盒。ACTH 小鼠血清稀釋5倍后樣本OD值在0.304-0.713之間。
By 羅老師
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