Chicken adrenocorticotropic hormone (ACTH) ELISA kit

產品詳情

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文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

雞促腎上腺皮質激素(ACTH)酶聯免疫試劑盒（CSB-E14373C）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ACTH含量。促腎上腺皮質激素（ACTH）是一種由腦垂體前葉分泌的多肽激素，主要作用是刺激腎上腺皮質分泌皮質醇，參與調節生物體的應激反應和晝夜節律。其分泌受下丘腦促腎上腺皮質激素釋放激素（CRH）調控，并通過負反饋機制維持激素水平的平衡。研究ACTH有助于理解內分泌系統的調節機制和疾病診斷。試劑盒檢測范圍為0.25 pg/mL-50 pg/mL，適用于家禽養殖業中應激生理研究、實驗室條件下激素分泌調控機制分析，以及疾病模型中腎上腺 - 垂體軸功能評估等科研場景。可為禽類健康管理、環境適應研究及藥理學實驗中 ACTH 水平監測提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

ACTH
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.25 pg/mL-50 pg/mL
靈敏度：

0.125 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-102
1:2	Average %	91
	Range %	97-95
1:4	Average %	89
	Range %	84-93
1:8	Average %	98
	Range %	92-104

回收率：

The recovery of chicken ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	91-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.