Hamster Interleukin-10(IL10) ELISA kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

倉鼠白介素10(IL10)酶聯(lián)免疫試劑盒（CSB-EL011580HA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL10含量。IL10是一種細胞因子，其背景在于它具有免疫調(diào)節(jié)作用，能抑制炎癥反應。研究機制上，它可作用于多種免疫細胞，通過與特異性受體結(jié)合，激活下游信號通路，調(diào)控細胞因子的產(chǎn)生與釋放，在免疫性疾病、感染等研究領(lǐng)域備受關(guān)注。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，IL10水平變化常被用于評估感染性疾病、自身免疫病等病理模型的免疫狀態(tài)；尤其適用于倉鼠炎癥模型研究、疫苗免疫效果評價或藥物干預實驗中的生物標志物分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
縮寫：

IL10
種屬：

Mesocricetus auratus (Golden hamster)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	91-105
1:2	Average %	106
	Range %	97-110
1:4	Average %	95
	Range %	87-99
1:8	Average %	93
	Range %	84-97

回收率：

The recovery of hamster IL10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-97
EDTA plasma (n=4)	95	90-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.