Bovine Interleukin 10,IL-10 ELISA Kit

Bovine Interleukin 10,IL-10 ELISA Kit

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文獻引用 (15) 說明書下載

中文名稱：

牛白介素10，IL-10酶聯免疫試劑盒
貨號：

CSB-E12917B
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

牛白介素10，IL-10酶聯免疫試劑盒（CSB-E12917B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL10含量。IL10，即白細胞介素-10，是一種具有抗炎和免疫調節功能的細胞因子。它主要由抗原遞呈細胞如活化的T細胞、單核細胞、B細胞和巨噬細胞分泌。IL10通過與其受體結合，激活JAK1和TYK2激酶，進而調節STAT1和STAT3信號通路，抑制Th1細胞因子產生，并抑制抗原呈遞細胞的細胞因子產生能力，發揮其抗炎作用。試劑盒檢測范圍為5 pg/mL-1000 pg/mL，適用于實驗室研究牛類炎癥相關疾病機制、疫苗免疫效果評估或畜牧領域健康監測等科研用途本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
縮寫：

IL10
Uniprot No.：

P43480
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

5 pg/mL-1000 pg/mL
靈敏度：

2.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-98
1:2	Average %	95
	Range %	91-98
1:4	Average %	96
	Range %	87-102
1:8	Average %	97
	Range %	91-104

回收率：

The recovery of bovine IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	95-100
EDTA plasma (n=4)	96	92-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.098	0.095	0.097
250	0.301	0.285	0.293
80	0.487	0.458	0.473
20	0.972	0.975	0.974
5	1.364	1.316	1.340
0	2.128	2.211	2.170

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

The supplementation of dietary black cumin (Nigella Sativa) seeds on performance, blood hematology, post-metabolic responses, antioxidant status, immunity, and inflammatory markers in pre-weaning calves M Elsayed, KM Al-Marakby, S Abdel Hafez,Tropical Animal Health and Production,2025
Dynamics of oxidative stress and immune responses in neonatal calves during diarrhea ZL Fu,Journal of dairy science,2024
Antimicrobial Resistance of Staphylococcus aureus and Coagulase?negative Staphylococci from Bovine Mastitis Milk with Detection of Interleukins in Milk and Serum of Infected Cows A Shehab,Journal of Advanced Veterinary Research,2023
The relationship between serum cytokine profile and vitamin D in calves with neonatal diarrhea M Caliskan,Cytokine,2023
Tropical study on Clinical and Host Cell Responses of Acute Phase Proteins, Pro-Inflammatory Cytokines and Antibodies’ in Lactating Dairy Cows Vaccinated and Challenged with S. aureus IU Hambali,ResearchGate,2022
Phyllanthus emblica (Amla) Fruit Powder as a Supplement to Improve Preweaning Dairy Calves’ Health: Effect on Antioxidant Capacity, Immune Response, and Gut Bacterial Diversity M Nguse,Biology,2022
Effect of non-encapsulated and encapsulated active dried yeast on blood cell count, blood metabolites and immune response of finishing beef heifers P Jiao,Canadian Journal of Animal Science ja,2021
In-vitro analysis of Interleukin-10 expression in cell cultures of Crossbred cattle, Tharparkar cattle and Murrah buffalo in response to mastitis causing antigens derived from Staphylococcus aureus and Escherichia coli Sourabh Sulabh, et al,Biological Rhythm Research,2019
Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows Hambali IU, et al,Tropical animal health and production,2018
Differential IFN-Gamma (IFN-γ), Interleukin 10 (IL-10) and Cardiac Troponin I (cTnI) Responses in Natural Bovine Trypanosomosis in Nigeria Michael I. Takeet1.et al,Open Journal of Veterinary Medicine,2016
Molecular and pathological studies on natural cases of bovine theileriosis Gupta A.et al,Journal of Parasitic Diseases,2016
Kinetics of selected plasma cytokines during innate-adaptive immune response transition in adult cattle infected with the bovine ephemeral fever virus R. Barigye.et al,Veterinary Microbiology,2016
Effect of in vitro zinc supplementation on HSPs expression and Interleukin 10 production in heat treated peripheral blood mononuclear cells of transition Sahiwal and Karan Fries cows Sheikh AA.et al,J Therm Biol.,2016
Kinetics of pro-inflammatory cytokines, interleukin-10, and virus neutralising antibodies during acute ephemeral fever virus infections in Brahman cattle Barigye R. et al,Vet Immunol Immunopathol,2015
Increased blood-circulating interferon-r, interleukin-17, and osteopontin levels in bovine paratuberculosis Dudemaine PL et al,J Dairy Sci,2014

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功能：

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
基因功能參考文獻：
1. Studied expression of Interleukin 10 (IL-10) in blood and milk samples of 25 healthy and 25 mastitic cows. Found IL-10 expression to be significantly higher in the blood and milk samples of mastitic cows compared to the healthy ones. PMID: 28267404
2. This study seems to indicate that PGE2 in cattle does not produce an anti-inflammatory effect by increasing the synthesis of IL-10. PMID: 25286662
3. IL-10 mRNA expression increased on day 8 in the mononuclear leukocytes of pregnant cows. In a cell culture experiment, interferon-tau stimulated stimulated IL-10 mRNA expression in mononuclear leukocytes. PMID: 22052007
4. Bovine IL-10 potently inhibits the activation of human myeloid cells in response to toll like receptor activation. PMID: 21464967
5. Levels in milk are altered in the presence of bacteria. PMID: 15372997
6. Monocytes obtained from cows with subclinical infection with MAP had upregulated expression of IL-10 and SOCS-3, which may have attenuated the capacity of mononuclear phagocytes to initiate inflammatory and adaptive immune responses. PMID: 16111147
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亞細胞定位：

Secreted.
蛋白家族：

IL-10 family
數據庫鏈接：

KEGG: bta:281246

STRING: 9913.ENSBTAP00000008793

UniGene: Bt.4723

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.