Chicken Interleukin 10,IL-10 ELISA Kit

Chicken Interleukin 10,IL-10 ELISA Kit

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中文名稱：

雞白介素10(IL-10)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E12835C
規(guī)格：

96T/48T
價(jià)格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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雞白介素10(IL-10)酶聯(lián)免疫試劑盒（CSB-E12835C）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL10含量。IL10是重要的細(xì)胞因子，具有抗炎特性，可抑制促炎細(xì)胞因子產(chǎn)生。其在維持機(jī)體免疫平衡中有關(guān)鍵作用。研究機(jī)制上，主要通過與受體結(jié)合，激活相關(guān)信號(hào)通路，抑制炎癥介質(zhì)釋放，在炎癥性疾病等研究中受廣泛關(guān)注。試劑盒檢測(cè)范圍為1 pg/mL-200 pg/mL，支持科研人員高效檢測(cè)雞感染性疾病模型、疫苗免疫效果評(píng)估或免疫調(diào)節(jié)機(jī)制研究中的IL - 10動(dòng)態(tài)變化。適用于家禽免疫學(xué)、病理學(xué)及生物制劑開發(fā)等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit
縮寫：

IL10
Uniprot No.：

Q6A2H4
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

1 pg/mL-200 pg/mL
靈敏度：

0.5 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-101
1:2	Average %	97
	Range %	94-105
1:4	Average %	95
	Range %	89-100
1:8	Average %	92
	Range %	87-96

回收率：

The recovery of chicken IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	97	93-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	0.155	0.152	0.154
50	0.334	0.348	0.341
16	0.515	0.502	0.509
4	0.969	0.975	0.972
1	1.436	1.463	1.450
0	2.658	2.667	2.663

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Impact of multistrain probiotics on growth performance, immune response, and gut morphometry in broiler chicken Gallus gallus domesticus S Younas, DA Bukhari, Z Bibi, A Ullah, A Rehman,Poultry Science,2025
Ameliorative effects of Sida acuta and vitamin C on serum DNA damage, pro-inflammatory and anti-inflammatory cytokines in roosters fed aflatoxin B1 contaminated diets OJ Olarotimi,Olarotimi O J,,2023
Production performance, immune indices, and caecal microbiota in broiler chickens fed diets supplemented with Morinda lucida leaf powder K Adeyemi,Tropical Agriculture,2023
Synergistic Immunosuppression of Avian Leukosis Virus Subgroup J and Infectious Bursal Disease Virus Is Responsible for Enhanced Pathogenicity W Chen,Viruses,2022
Influence of Dietary Supplementation of Guava Leaf, Oxytetracycline, and Tert-Butylhydroxytoluene on Growth Performance, Gut Microbial Population, Immune?… KD Adeyemi,Islamic Azad University,2022
Hydroxy-Selenomethionine Improves the Selenium Status and Helps to Maintain Broiler Performances under a High Stocking Density and Heat Stress Conditions through a Better Redox and Immune Response H Sun,Antioxidants,2021
Evaluation of immunoprotective effects of recombinant protein and DNA vaccine based on Eimeria tenella surface antigen 16 and 22 in vivo Rui Fang,Parasitology Research,2021
Influence of Anacardium occidentale leaf supplementation in broiler chicken diet on performance, caecal microbiota, blood chemistry, immune status, carcass, and meat quality Foluke Sola-Ojo,British Poultry Science,2021
In vivo immunoprotective comparison between recombinant protein and DNA vaccine of Eimeria tenella surface antigen 4 P Zhao,Veterinary Parasitology,2020
A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine Liu Y, et al,Journal of Immunology Research,2019
Egg White/Egg Shell based Biomimetic Hybrid Hydrogels for Bone Regeneration Keqing Huang, et al,Acs Biomaterials-Science & Engineering,2019
Effects of sodium butyrate on intestinal health and gut microbiota composition during intestinal inflammation progression in broilers Zou X,Poultry Science,2019
Oxidative Stress in Microbial Diseases: Pathogen, Host, and Therapeutics Novaes RD, et al,Oxidative Medicine And Cellular Longevity,2019
Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers Shahid Ali Rajput, et al,Toxins,2019
Xylanase and Fermented Polysaccharide of Hericium caputmedusae Reduce Pathogenic Infection of Broilers by Improving Antioxidant and Anti-Inflammatory Properties Sitong Zhang, et al,Oxidative Medicine and Cellular Longevity,2018
The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,2018
Dextran sulphate sodium (DSS) causes intestinal histopathology and inflammatory changes consistent with increased gut leakiness in chickens Zou X.et al,Br Poult Sci,2017
Effects of yeast cell wall on growth performance, immune responses and intestinal short chain fatty acid concentrations of broiler in an experimental necrotic enteritis model Guang-DaXue.et al,Animal Nutrition,2017
The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,2016
The molecular characterization and immune protection of microneme 2 of Eimeria acervulina ZC Zhang. et al,Veterinary Parasitology,2015
Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015
Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge Huang J. et al,Parasitol Int,2015
Panax notoginseng saponins mitigate ovariectomy-induced bone loss and inhibit marrow adiposity in rats. Fan JZ. et al,Menopause.,2015

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最新研究進(jìn)展：

IL-10是一種抗炎因子，對(duì)調(diào)節(jié)炎癥反應(yīng)和維持免疫平衡具有重要作用。最新研究發(fā)現(xiàn)，IL-10參與許多自身免疫性疾病的發(fā)病機(jī)制，并且IL-10的水平可能作為這些疾病的生物標(biāo)記物。此外，IL-10在腫瘤微環(huán)境中也發(fā)揮作用，可以通過抑制炎癥反應(yīng)和免疫應(yīng)答來促進(jìn)腫瘤生長。
功能：

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
亞細(xì)胞定位：

Secreted.
蛋白家族：

IL-10 family
組織特異性：

Expressed predominantly in bursa of Fabricius and cecal tonsils with low levels in thymus, liver and lung.
數(shù)據(jù)庫鏈接：

KEGG: gga:428264

STRING: 9031.ENSGALP00000001310

UniGene: Gga.46641

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.