PODXL

PODXL，全名為Podocalyxin-like，常見別名為CD34相關(guān)蛋白。該蛋白主要表達(dá)于血管內(nèi)皮細(xì)胞，具有調(diào)節(jié)細(xì)胞粘附和遷移的功能。PODXL通過影響細(xì)胞表面電荷分布，參與血管的穩(wěn)定性和通透性調(diào)節(jié)。在生理狀態(tài)下，它有助于維持正常的血管結(jié)構(gòu)和功能。然而，當(dāng)PODXL表達(dá)異常時(shí)，可能與多種疾病的發(fā)生發(fā)展相關(guān)，如癌癥轉(zhuǎn)移、動(dòng)脈粥樣硬化等。近年來，針對(duì)PODXL的藥物研發(fā)取得了一定進(jìn)展，例如通過抑制PODXL的表達(dá)或活性，來阻止腫瘤細(xì)胞的侵襲和轉(zhuǎn)移。此外，一些抗血管生成藥物也顯示出與PODXL相互作用的潛力，為相關(guān)疾病的治療提供了新的思路。

熱銷產(chǎn)品

Recombinant Human Podocalyxin (PODXL), partial (CSB-YP518830HU)

驗(yàn)證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP518830HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PODXL.

PODXL Monoclonal Antibody (CSB-MA134471A0m)

驗(yàn)證數(shù)據(jù)

Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate
All lanes: PODXL antibody at 2.5µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: PODXL antibody at 2.5µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: PODXL antibody at 0.43µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Western Blot
Positive WB detected in: Rat brain tissue, Rat heart tissue, Rat kidney tissue
All lanes: PODXL antibody at 1.28µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA134471A0m at dilution of 1:200

Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA134471A0m at dilution of 1:200

Immunofluorescent analysis of Hela cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of PC-3 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of A549 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of Ntera-2 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Overlay histogram showing Hela cells stained with CSB-MA134471A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*10⁶cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IGg2b (10µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunoprecipitating PODXL in HEK293 whole cell lysate
Lane 1: Mouse control IgG (1µg) instead of CSB-MA134471A0m in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA134471A0m (8µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (10µg)

PODXL Antibody

PODXL Protein

PODXL ELISA Kit

PODXL Antibodies

PODXL for Homo sapiens (Human)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA13447A0Rb	PODXL Antibody	Human	ELISA, WB, IHC, IF
CSB-PA13447B0Rb	PODXL Antibody, HRP conjugated	Human	ELISA
CSB-PA13447C0Rb	PODXL Antibody, FITC conjugated	Human
CSB-PA13447D0Rb	PODXL Antibody, Biotin conjugated	Human	ELISA
CSB-MA134471A0m	PODXL Monoclonal Antibody	Human, Mouse, Rat	ELISA, WB, IHC, IP, IF, FC
CSB-PA568085	PODXL Antibody	Human	ELISA,IHC
CSB-PA174197	PODXL Antibody	Human	ELISA,IHC

PODXL Proteins

PODXL Proteins for Homo sapiens (Human)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP518830HU	Recombinant Human Podocalyxin (PODXL), partial	Yeast
CSB-EP518830HU	Recombinant Human Podocalyxin (PODXL), partial	E.coli

PODXL Proteins for Gallus gallus (Chicken)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP527565CH CSB-EP527565CH CSB-BP527565CH CSB-MP527565CH CSB-EP527565CH-B	Recombinant Chicken Podocalyxin (PODXL), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

PODXL Proteins for Oryctolagus cuniculus (Rabbit)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP632978RB CSB-EP632978RB CSB-BP632978RB CSB-MP632978RB CSB-EP632978RB-B	Recombinant Rabbit Podocalyxin (PODXL), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

PODXL Proteins for Canis familiaris (Dog) (Canis lupus familiaris)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP684440DO CSB-EP684440DO CSB-BP684440DO CSB-MP684440DO CSB-EP684440DO-B	Recombinant Dog Podocalyxin (PODXL), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

PODXL Proteins for Mus musculus (Mouse)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP865629MO CSB-EP865629MO CSB-BP865629MO CSB-MP865629MO CSB-EP865629MO-B	Recombinant Mouse Podocalyxin (Podxl), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

PODXL Proteins for Rattus norvegicus (Rat)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP893876RA	Recombinant Rat Podocalyxin (Podxl), partial	Yeast
CSB-EP893876RA CSB-BP893876RA CSB-MP893876RA CSB-EP893876RA-B	Recombinant Rat Podocalyxin (Podxl), partial	E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli