Rat Podocalyxin,PCX ELISA Kit

Rat Podocalyxin,PCX ELISA Kit

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中文名稱：

大鼠足細胞標記蛋白/足盂蛋白(PCX)酶聯免疫試劑盒
貨號：

CSB-E13946r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠足細胞標記蛋白/足盂蛋白(PCX)酶聯免疫試劑盒（CSB-E13946r）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織培養(yǎng)上清液樣本中的PODXL含量。PODXL即足糖萼蛋白，它是一種跨膜蛋白，在胚胎發(fā)育等過程發(fā)揮作用。研究顯示它與腫瘤發(fā)生、發(fā)展密切相關，可促進腫瘤細胞增殖、遷移和侵襲，還能影響腫瘤血管生成，有望成為癌癥診斷標志物和治療靶點，為癌癥防治提供新思路。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，科研人員可通過動態(tài)監(jiān)測實驗動物或細胞模型中PCX的表達變化，用于探究腎臟疾病發(fā)生發(fā)展機制、評估藥物干預效果或建立體外足細胞損傷模型等基礎研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Podxl ELISA Kit; Pclp1 ELISA Kit; Podocalyxin ELISA Kit; Podocalyxin-like protein 1 ELISA Kit; PC ELISA Kit; PCLP-1 ELISA Kit
縮寫：

PODXL
Uniprot No.：

Q9WTQ2
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, urine, cell culture supernates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PCX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-93
1:2	Average %	97
	Range %	93-101
1:4	Average %	89
	Range %	85-93
1:8	Average %	98
	Range %	92-104

回收率：

The recovery of rat PCX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-97
EDTA plasma (n=4)	89	83-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.201	2.312	2.257	2.112
50	1.574	1.456	1.515	1.370
25	1.009	1.056	1.033	0.888
12.5	0.633	0.654	0.644	0.499
6.25	0.401	0.412	0.407	0.262
3.12	0.272	0.286	0.279	0.134
1.56	0.210	0.215	0.213	0.068
0	0.144	0.146	0.145

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Rat pancreatectomy combined with isoprenaline or uninephrectomy as models of diabetic cardiomyopathy or nephropathy L Thisted,Scientific Reports,2020
Effects of FoxO1 on podocyte injury in diabetic rats Guo F. et al,Biochem Biophys Res Commun,2015

功能：

Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
基因功能參考文獻：
1. podocalyxin has a role in defining the structure of the podocyte basal surface PMID: 15226400
2. Podocalyxin might be associated with the malignant progression of astrocytic tumors, and be a useful prognostic marker for astrocytic tumors. PMID: 18639524
3. Report ubiquitous yet distinct expression of podocalyxin on vascular surfaces in normal and tumor tissues in the rat. PMID: 19142011
亞細胞定位：

Apical cell membrane. Cell projection, microvillus. Membrane raft. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Membrane; Single-pass type I membrane protein.
蛋白家族：

Podocalyxin family
組織特異性：

Glomerular epithelium cell (podocyte) (at protein level).
數據庫鏈接：

KEGG: rno:192181

STRING: 10116.ENSRNOP00000016991

UniGene: Rn.162741

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.