CA1
CA1，即碳酸酐酶1，別名CAB/CAR1/CAN等。它是一種膜結(jié)合糖蛋白，屬于碳酸酐酶相關(guān)蛋白家族成員，主要在人體細(xì)胞的質(zhì)膜上表達(dá)，尤其在肺、腎、前列腺和紅細(xì)胞中表達(dá)量較高。CA1的作用機(jī)制是通過(guò)催化二氧化碳（CO2）和水（H2O）之間的可逆反應(yīng)，生成碳酸氫根離子（HCO3-）和質(zhì)子（H+），從而參與調(diào)節(jié)體內(nèi)酸堿平衡和氣體交換。這一過(guò)程對(duì)于維持血液pH值和促進(jìn)氧氣釋放至組織至關(guān)重要。CA1在生物學(xué)上的意義非常廣泛，它參與了多種生理過(guò)程，包括呼吸、代謝和酸堿平衡的維持。在肺中，CA1幫助調(diào)節(jié)氣體交換，促進(jìn)CO2的排出；在腎中，它參與碳酸氫鹽的重吸收，影響尿液的酸化；在前列腺中，CA1與腫瘤的酸化微環(huán)境有關(guān)，可能影響腫瘤細(xì)胞的增殖和轉(zhuǎn)移。此外，CA1的活性還與某些遺傳性疾病相關(guān)，例如，某些形式的II型碳酸酐酶缺乏癥與SLC45A1基因突變有關(guān)。因此，CA1不僅在維持生理功能中發(fā)揮關(guān)鍵作用，也是醫(yī)學(xué)研究和治療干預(yù)的潛在靶點(diǎn)。
熱銷產(chǎn)品
Recombinant Human Carbonic anhydrase 1 (CA1) (CSB-EP004364HU)
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
CA1 Recombinant Monoclonal Antibody (CSB-RA217389A0HU)
驗(yàn)證數(shù)據(jù)
Western Blot
Positive WB detected in: K562 whole cell lysate,U937 whole cell lysate
All lanes: Ikaros antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
IHC image of CSB-RA217389A0HU diluted at 1:50 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.36% DAB.
Overlay Peak curve showing MCF-7 cells stained with CSB-RA217389A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
CA1 Antibodies
CA1 for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA004364GA01HU | CA1 Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA004364HC01HU | CA1 Antibody, FITC conjugated | Human | |
| CSB-PA004364HA01HU | CA1 Antibody | Human, Mouse | ELISA, WB, IHC, IF, IP |
| CSB-PA004364ESR1HU | CA1 Antibody | Human, Mouse | ELISA, WB, IHC |
| CSB-PA004364ESR2HU | CA1 Antibody | Human | ELISA, IHC |
| CSB-PA545638 | CA1 Antibody | Human | ELISA,WB,IHC |
| CSB-PA060110 | CA1 Antibody | Human | WB, IHC, ELISA |
| CSB-PA348998 | CA1 Antibody | Human,Rat | ELISA,WB |
| CSB-PA910869 | CA1 Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-RA217389A0HU | CA1 Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC, FC |
CA1 Proteins
CA1 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP004364HU | Recombinant Human Carbonic anhydrase 1 (CA1) | Yeast |
| CSB-EP004364HU | Recombinant Human Carbonic anhydrase 1 (CA1) | E.coli |
| CSB-BP004364HU CSB-MP004364HU CSB-EP004364HU-B |
Recombinant Human Carbonic anhydrase 1 (CA1) | Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-AP005391HU | Recombinant Human Carbonic anhydrase 1 (CA1) (Active) | E.coli |
CA1 ELISA Kit
CA1 ELISA Kit for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-EL004364HU | Human Carbonic anhydrase 1(CA1) ELISA kit | serum, plasma, tissue homogenates | 0.78 pg/mL |
