CA1 Recombinant Monoclonal Antibody

說明書

中文名稱：

CA1重組抗體
貨號：

CSB-RA217389A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: K562 whole cell lysate,U937 whole cell lysate
All lanes: Ikaros antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa

IHC image of CSB-RA217389A0HU diluted at 1:50 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.36% DAB.

Overlay Peak curve showing MCF-7 cells stained with CSB-RA217389A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
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靶點詳情

產品詳情

產品描述：

CA1 Recombinant Monoclonal Antibody（CSB-RA217389A0HU）是一款針對碳酸酐酶1（CA1）的高特異性科研工具，適用于多種實驗場景。CA1作為碳酸酐酶家族成員，廣泛參與酸堿平衡調節及二氧化碳代謝過程，其表達異常與代謝性疾病、腫瘤微環境調控等研究領域密切相關。本抗體經ELISA、Western Blot（WB）、免疫組織化學（IHC）和流式細胞術（FC）嚴格驗證：在WB中可檢測到清晰靶蛋白條帶，推薦稀釋比例1:500-1:2000；IHC實驗顯示其在石蠟包埋組織中具有明確的亞細胞定位能力，推薦使用濃度1:50-1:200；FC驗證證實其適用于細胞表面或胞內CA1蛋白的檢測，建議稀釋范圍1:50-1:200。該抗體可應用于細胞代謝機制研究、疾病模型構建及組織病理學分析等科研方向，其重組單克隆特性確保了批次間的高度一致性，并經過抗原親和純化以降低非特異性背景。實驗時建議根據樣本類型優化具體條件，配套設立陽性和陰性對照以確保結果可靠性。
Uniprot No.：

P00915
基因名：

CA1
別名：

Carbonic anhydrase 1 (EC 4.2.1.1) (Carbonate dehydratase I) (Carbonic anhydrase B) (CAB) (Carbonic anhydrase I) (CA-I), CA1
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human CA1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

26E3
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.
基因功能參考文獻：
1. Anti-CA I and II antibody levels were investigated using ELISA in serum samples from 30 patients with Acute Myeloid Leukemia (AML) and 30 healthy peers. Anti-CA I and II antibody titers in the AML group were significantly higher compared with the control group (p=0.0001 and 0.018, respectively). PMID: 28270370
2. These six-membered carbocycles showed nice inhibitory action against AChE and human carbonic anhydrase (hCA) II and I isoforms. The hCA I, II, and AChE were efficiently inhibited by these molecules, with Ki values in the range of 6.70-35.85 nM for hCA I, 18.77-60.84 nM for hCA II, and 0.74-4.60 for AChE, respectively PMID: 28613396
3. These results showed that flavonoids especially malvin and oenin effectively inhibited hCA I and II isoenzymes. PMID: 28445001
4. Discovery of potent carbonic anhydrase, acetylcholinesterase, and butyrylcholinesterase enzymes inhibitors: The new amides and thiazolidine-4-ones synthesized on an acetophenone base. PMID: 28544359
5. Here are presented solved the first crystal structures of carbonic anhydrase 1and carbonic anhydrase 2 in complex with polmacoxib, at 2.0 A and 1.8 A, respectively. PMID: 27475498
6. Neuronal carbonic anhydrase I (CA1) appears to be associated with the endoplasmic reticulum subcellular structure. PMID: 27809276
7. CA1 is highly expressed in the sera of stage I non-small cell lung cancer patients PMID: 26232327
8. We demonstrated that resveratrol, caffeic acid, and tannic acid in stored blood could decrease the sensitivity to oxidation of erythrocytes in vitro but did not exhibit such effects on Carbonic Anhydrase activity. PMID: 27413740
9. CA1 is a potential oncogene and that it contributes to abnormal cell calcification, apoptosis and migration in breast cancer. PMID: 26459317
10. We report on the competitive zinc metalation of apo-carbonic anhydrase [CA; metal-free CA (apo-CA)] in the presence of apo-metallothionein 1A domain fragments to identify domain specific determinants of zinc binding and zinc donation PMID: 26475450
11. The gene encoding CA1 is susceptible to ankylosing spondylitis and plays a role in the process of bone formation. PMID: 22838845
12. Structure-activity relationships study showed that indolylchalcone derivatives have higher inhibitory activities than pyrido[2,3-d]pyrimidine derivatives on hCA I and hCA II. PMID: 25165709
13. Myocardial carbonic anhydrase 1/2 activation is significantly elevated in diabetic ischemic cardiomyopathy. PMID: 24670789
14. may be involved in the pathogenesis of Abdominal aortic aneurysm PMID: 23557951
15. The new prognostic biomarkers GRP78, Fructose-bisphosphate Aldolase A (ALDOA), Carbonic Anhydrase I (CA1) and Peptidyl-prolyl cis-trans isomerase A or Cyclophilin A (PPIA)) provided good survival prediction for TNM stage I-IV patients. PMID: 22996014
16. A significant correlation was found between positive carbonic anhydrase I staining and oral squamous cell carcinoma for more advanced clinical stage and larger tumor size, but not for positive lymph node metastasis, distal metastasis, and recurrence. PMID: 22416960
17. carbonic anhydrase I, phosphoglycerate kinase 1 and apolipoprotein A-I appeared to be the most significant variations of proteins in patients with osteopenia and osteoarthritis PMID: 22619369
18. A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration. PMID: 22360420
19. Overexpression of CA1 in the synovial tissues of ankylosing spondylitis (AS) patients may promote improper calcification and bone resorption in AS. PMID: 21143847
20. single mutation of an amino acid not considered essential to catalysis (Phe91Asn) in carbonic anhydrase I, but is near substrate binding pocket, led to increase of catalytic activity; mutant CA I also showed higher affinity for sulfonamide inhibitors PMID: 20624682
21. The X-ray crystallographic structure of the CA I Michigan 1 variant isozyme is reported, both in the presence and absence of a second bound zinc ion coordinated to His 64, His 200, and Arg 67. PMID: 12009884
22. concentrations in nondialyzed chronic kidney disease patients, and the relationship with acidosis, zinc, anemia, and iron supplementation PMID: 14675565
23. cytosolic CA I, II, and XIII are downregulated in neoplastic colorectal mucosa compared to normal colorectal mucosa PMID: 15836783
24. Isothiocyanato sulfonamide thioureas inhibit this enzyme. PMID: 15837325
25. X-ray crystallographic structure for the adduct of an activator with hCA I. Binding site interactions of activator L-Histidine with active site amino acids PMID: 16870440
26. study presents the X-ray structure of the foscarnet adduct with CA1 together with the factors governing recognition of such small molecules by a metalloenzyme active site PMID: 17314045
27. Decreased levels of carbonic anhydrase 1 isozyme is associated with type II diabetes. PMID: 17464559
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亞細胞定位：

Cytoplasm.
蛋白家族：

Alpha-carbonic anhydrase family
數據庫鏈接：

HGNC: 1368

OMIM: 114800

KEGG: hsa:759

STRING: 9606.ENSP00000256119

UniGene: Hs.23118

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