ADAM8
ADAM8，全稱為A Disintegrin and metalloproteinase domain-containing protein 8，是一種在人類中由ADAM8基因編碼的酶。它也被稱為CD156、CD156a、MS2或ADAM metallopeptidase domain 8。ADAM8屬于ADAMs家族成員，具有蛋白水解酶活性，能夠水解并釋放多種細胞表面蛋白的胞外功能區，進而影響多種細胞因子功能并參與細胞外基質的降解。ADAM8的表達最初發現于巨噬細胞，隨后相繼發現在人B淋巴細胞、樹突狀細胞、神經膠質細胞及人嗜酸性粒細胞等細胞內均有表達。
ADAM8的異常表達與多種惡性腫瘤的轉移密切相關，如肺癌、乳腺癌、胰腺癌、肝癌等。研究表明，ADAM8過表達可減弱腫瘤細胞間的黏附作用，并促進細胞外基質降解和細胞膜上細胞因子的釋放，同時有助于腫瘤部位新生血管生成，從而促進了腫瘤細胞的轉移。因此，ADAM8成為惡性腫瘤的預后指標和潛在治療靶點，備受關注。目前，ADAM8在腫瘤治療中的應用前景主要有兩個方面：一是作為生物標志物，檢測ADAM8的表達可以反映腫瘤狀態，有效預測患者預后；二是作為藥物設計的靶標，使用ADAM8選擇性抑制劑抑制ADAM8可能會產生較好的治療作用。
熱銷產品
ADAM8 Antibody (CSB-PA10259A0Rb)
驗證數據
Western Blot
Positive WB detected in: THP-1 whole cell lysate(20μg), K562 whole cell lysate(20μg), JK whole cell lysate(20μg)
All lanes: ADAM8 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 89, 79,81 kDa
Observed band size: 100 kDa
Exposure time: 300s
ImmunofluorescencestainingofHelacellwithCSB-PA10259A0Rbat1:30,counter-stainedwithDAPI.Thecellswerefixedin4%formaldehydeandblockedin10%normalGoatSerum.Thecellswerethenincubatedwiththeantibodyovernightat4C.ThesecondaryantibodywasAlexaFluor488-congugatedAffiniPureGoatAnti-RabbitIgG(H+L).
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of MCF-7 cell with CSB-PA10259A0Rb at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ImmunofluorescencestainingofMCF-7cellwith5%goatserum,counter-stainedwithDAPI.Thecellswerefixedin4%formaldehydeandblockedin10%normalGoatSerum.Thecellswerethenincubatedwiththeantibodyovernightat4C.ThesecondaryantibodywasAlexaFluor488-congugatedAffiniPureGoatAnti-RabbitIgG(H+L).
ADAM8 Antibodies
ADAM8 for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA10259A0Rb | ADAM8 Antibody | Human | ELISA, WB, IF |
| CSB-PA10259B0Rb | ADAM8 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA10259C0Rb | ADAM8 Antibody, FITC conjugated | Human | |
| CSB-PA10259D0Rb | ADAM8 Antibody, Biotin conjugated | Human | ELISA |
ADAM8 Proteins
ADAM8 Proteins for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP001295HU CSB-EP001295HU CSB-BP001295HU CSB-MP001295HU CSB-EP001295HU-B |
Recombinant Human Disintegrin and metalloproteinase domain-containing protein 8 (ADAM8), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
