Recombinant Escherichia coli Protein-export membrane protein SecG (secG)

in vitro E.coli expression system

中文名稱：

大腸桿菌secG重組蛋白
貨號：

CSB-CF314852ENV
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

secG
Uniprot No.：

P0AG99
別名：

secG; b3175; JW3142; Protein-export membrane protein SecG; P12; Preprotein translocase band 1 subunit
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達區域：

1-110
氨基酸序列

MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKPTSDIPN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Subunit of the protein translocation channel SecYEG. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true it also results in FtsH-mediated degradation of SecY. Treatment with antibiotics that block translation elongation such as chloramphenicol also leads to degradation of SecY and SecE but not SecG.
基因功能參考文獻：
1. Authors show that SecG plays a critical role for the residual export mediated by mutant signal sequences and that the SecYE dimer can often be detected only in the presence of SecG. PMID: 25404704
2. The hydrophobicity threshold for SecYEG-mediated cell membrane insertion is distinctly lower than that for Sec61-mediated insertion. PMID: 23659793
3. Data show that ribosome binding is sufficient to open the SecYEG channel. PMID: 23645666
4. Data indicate taht SecG inversion occurs only when MPIase associates with SecYEG. PMID: 23716687
5. Charge composition features of model single-span membrane proteins that determine selection of YidC and SecYEG translocase pathways in Escherichia coli PMID: 23355473
6. analysis of conformational dynamics of the plug domain of the SecYEG protein-conducting channel PMID: 22033919
7. SecYEG-bound SecA undergoes ATP-dependent conformational changes that are not observed for soluble SecA. PMID: 15850376
8. cryo-electron microscopy reconstruction of the E. coli protein-conducting channel, SecYEG, complexed with the ribosome and a nascent chain containing a signal anchor PMID: 16292303
9. cotranslational targeting of a protein to the E. coli SecYEG complex does not ensure its export but that export also depends on a subsequent event that involves sequences both within and just beyond the signal peptide PMID: 19211555
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亞細胞定位：

Cell inner membrane; Multi-pass membrane protein.
蛋白家族：

SecG family
數據庫鏈接：

KEGG: ecj:JW3142

STRING: 316385.ECDH10B_3349