Recombinant Mouse Interleukin-1 receptor accessory protein (Il1rap), partial (Active)

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Stock}

貨號：

CSB-MP730751MO
說明書：

MSDS Datasheet
規格：

￥696
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Il1rap protein at 2 μg/ml can bind Anti-Il1RAP recombinant antibody (CSB-RA878844MA2HU). The EC₅₀ is 2.279-3.955 ng/mL. Biological Activity Assay
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靶點詳情

產品詳情

純度：

Greater?than?95%?as?determined?by?SDS-PAGE.
內毒素：

Less?than?1.0?EU/ug?as?determined?by?LAL?method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Mouse Il1rap protein at 2 μg/mL can bind Anti-Il1RAP recombinant antibody (CSB-RA878844MA2HU). The EC50 is 2.279-3.955 ng/mL.
基因名：

Il1rap
Uniprot No.：

Q61730
別名：

Interleukin-1 receptor accessory protein; EC:3.2.2.6; IL-1 receptor accessory protein; IL-1RAcP; Interleukin-33 receptot beta chain; Il1rap
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

41.4 kDa
表達區域：

21-367aa
氨基酸序列

SERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKYNYSTAHSSGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSAMRFPVHKMYIEHGIHKITCPNVDGYFPSSVKPSVTWYKGCTEIVDFHNVLPEGMNLSFFIPLVSNNGNYTCVVTYPENGRLFHLTRTVTVKVVGSPKDALPPQIYSPNDRVVYEKEPGEELVIPCKVYFSFIMDSHNEVWWTIDGKKPDDVTVDITINESVSYSSTEDETRTQILSIKKVTPEDLRRNYVCHARNTKGEAEQAAKVKQKVIPPRYTVELACGFGAT
蛋白標簽：

C-terminal 10xHis-tagged
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized?from?a?0.2?μm?filtered?PBS,?6%?Trehalose,?pH?7.4
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Coreceptor for IL1RL2 in the IL-36 signaling system. Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD. May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells.; Associates with secreted ligand-bound IL1R2 and increases the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Enhances the ability of secreted IL1R1 to inhibit IL-33 signaling.; Required for Src phosphorylation by IL1B. Required for IL1B-potentiated NMDA-induced calcium influx in neurons acting in cooperation with IL1R1 isoform 2 to mediate Akt kinase activation.
基因功能參考文獻：
1. These results thus reveal the decoding mechanism of splice-insert signaling codes for synaptic differentiation induced by trans-synaptic adhesion between PTPdelta and IL1RAPL1/IL-1RAcP. PMID: 25908590
2. We conclude that neuron-specific AcPb plays a critical role in host defenses and sleep homeostasis. PMID: 25449578
3. In experimental biliary atresia, miR-29a/29b1 are upregulated, and reporter assays confirmed that Igf1 and Il1RAP are down-regulated by miR-29. PMID: 22167021
4. The results of this study suggested that IL-1RAcP represents an interesting molecular link between immune systems and synapse formation in the brain. PMID: 22357843
5. IL-1RAcP is essential for physiological activities of peripheral IL-1. PMID: 11847485
6. Identification of essential regions in the cytoplasmic tail of interleukin-1 receptor accessory protein critical for interleukin-1 signaling PMID: 11880380
7. determination of interaction sites in vitro mutagenesis and molecular modeling PMID: 13679373
8. data suggest that domain III of IL-1RAcP may be involved in the formation or stabilization of the IL-1RI/IL-1 complex by binding to epitopes on domain III of the IL-1RI created following IL-1 binding to the IL-1RI PMID: 17669273
9. IL-33 and ST2 form a complex with IL-1R accessory protein (IL-1RAcP), a signaling receptor subunit that is also a member of the IL-1R complex. PMID: 17675517
10. IL-1RAcP is used by more than one alpha-chain of the IL-1 receptor family and thus may resemble a common beta-chain of that family PMID: 18003919
11. these observations establish AcP as co-receptor for IL-33 signaling via ST2 and suggest a novel role for sAcP in modulating the biological activity of IL-33 PMID: 18450470
12. an isoform of the IL-1 receptor accessory protein (termed AcPb) was identified that is expressed exclusively in the CNS. PMID: 19481478
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亞細胞定位：

[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
蛋白家族：

Interleukin-1 receptor family
組織特異性：

Detected in lung, brain, spleen, thymus and liver. Expressed in brain endothelial cells, astrocytes, microglia and neurons. Isoform 3 is predominantly expressed in brain; expressed in hippocampal neurons.
數據庫鏈接：

KEGG: mmu:16180

UniGene: Mm.253424