Recombinant Human Translation factor GUF1, mitochondrial (GUF1)

中文名稱：

人GUF1重組蛋白
貨號：

CSB-YP822701HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人GUF1重組蛋白
貨號：

CSB-EP822701HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人GUF1重組蛋白
貨號：

CSB-EP822701HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人GUF1重組蛋白
貨號：

CSB-BP822701HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人GUF1重組蛋白
貨號：

CSB-MP822701HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

GUF1
Uniprot No.：

Q8N442
別名：

GUF1; Translation factor GUF1; mitochondrial; EC 3.6.5.-; Elongation factor 4 homolog; EF-4; GTPase GUF1; Ribosomal back-translocase
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
表達區域：

50-669
氨基酸序列

S SAEFKEKLDM SRFPVENIRN FSIVAHVDHG KSTLADRLLE LTGTIDKTKN NKQVLDKLQV ERERGITVKA QTASLFYNCE GKQYLLNLID TPGHVDFSYE VSRSLSACQG VLLVVDANEG IQAQTVANFF LAFEAQLSVI PVINKIDLKN ADPERVENQI EKVFDIPSDE CIKISAKLGT NVESVLQAII ERIPPPKVHR KNPLRALVFD STFDQYRGVI ANVALFDGVV SKGDKIVSAH TQKTYEVNEV GVLNPNEQPT HKLYAGQVGY LIAGMKDVTE AQIGDTLCLH KQPVEPLPGF KSAKPMVFAG MYPLDQSEYN NLKSAIEKLT LNDSSVTVHR DSSLALGAGW RLGFLGLLHM EVFNQRLEQE YNASVILTTP TVPYKAVLSS SKLIKEHREK EITIINPAQF PDKSKVTEYL EPVVLGTIIT PDEYTGKIMM LCEARRAVQK NMIFIDQNRV MLKYLFPLNE IVVDFYDSLK SLSSGYASFD YEDAGYQTAE LVKMDILLNG NTVEELVTVV HKDKAHSIGK AICERLKDSL PRQLFEIAIQ AAIGSKIIAR ETVKAYRKNV LAKCYGGDIT RKMKLLKRQA EGKKKLRKIG NVEVPKDAFI KVLKTQSSK
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.
基因功能參考文獻：
1. Homozygous variant in the GUF1 gene identified in the three siblings with West syndrome. PMID: 26486472
2. The structure provides insights into the tRNA-remodeling function of EF-4 on the ribosome and suggests that the displacement of the CCA-end of the A-site tRNA away from the peptidyl transferase center (PTC) is functionally significant. PMID: 27092003
相關疾病：

Epileptic encephalopathy, early infantile, 40 (EIEE40)
亞細胞定位：

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
蛋白家族：

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, LepA subfamily
數據庫鏈接：

HGNC: 25799

OMIM: 617064

KEGG: hsa:60558

STRING: 9606.ENSP00000281543

UniGene: Hs.546419