Recombinant Human CAMPATH-1 antigen (CD52)

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中文名稱：

Recombinant Human CAMPATH-1 antigen (CD52)
貨號：

CSB-EP004943HU
說明書：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
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產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

CD52
Uniprot No.：

P31358
別名：

Cambridge pathology 1 antigen; CAMPATH 1; CAMPATH 1 antigen; CAMPATH-1 antigen; CD 52; CD52; CD52 antigen; CD52 molecule; CD52_HUMAN; CDw52; CDW52 antigen; Epididymal secretory protein E5; Epididymis secretory sperm binding protein Li 171mP; HE 5; He5; Human epididymis-specific protein 5
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

5.2 kDa
表達區(qū)域：

25-36aa
氨基酸序列

GQNDTSQTSSPS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer,50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

The recombinant human CD52 protein is encoded by a recombinant DNA that was cloned into the expression vector and then transformed into the E.coli that supports the expression of the gene. The recombinant DNA was constructed by fusing the N-terminal 6xHis tag gene to the gene fragment coding for the 25-36aa of the human CD52 protein. After purification, the product is the recombinant human CD52 protein. This recombinant CD52 protein was subjected to the SDS-PAGE determination. Its purity reaches over 90% evaluated by Bandscan software analysis combined with SAS-PAGE. This CD52 protein ran to the molecular weight of about 20 kDa under SDS-PAGE condition.
CD52 is called a glycoprotein, which often is expressed in the surface of mature lymphocytes, monocyte or dendritic cells. CD52 protein consists of 12 amino acids, which anchored to glycosylphosphatidylinositol (GPI). It also acts to restrain the cells adhesion, which contributes to the freely move of cells. Studies implied that CD52 is related to some types of lymphoma. CD52 Is elevated on B cells of systemic lupus erythematosus (SLE) patients and regulates B cell function. The CD52 targeted agent alemtuzumab has been on the market to improve the treatment of chronic lymphocytic leukemia.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
基因功能參考文獻：
1. soluble CD52 exerts a concerted immunosuppressive effect by first sequestering HMGB1 to nullify its proinflammatory Box B, followed by binding to the inhibitory Siglec-10 receptor, triggering recruitment of SHP1 to the intracellular immunoreceptor tyrosine-based inhibitory motif of Siglec-10 and its interaction with the TCR. PMID: 29997173
2. CD52 is a novel prognostic NSC marker and a potential NSC target in a subset of patients with MDS and AML, which may have clinical implications and may explain clinical effects produced by alemtuzumab in these patients. PMID: 24799522
3. carbohydrate moiety of sperm protein interferes with the complement system via binding to C1q PMID: 22386526
4. CT60 single-nucleotide polymorphism of CTLA4 is a surrogate marker for donor lymphocyte infusion outcome after allogeneic cell transplantation for acute leukemia PMID: 21552305
5. Clonal large granular lymphocytes exhibited decreased CD52 expression post-therapy in patients refractory to treatment. PMID: 19794084
6. Our bioinformatics findings suggest that CD52 polymorphism may affect the efficiency of GPI anchor formation and thus may indirectly alter the response to anti-CD52 agents like alemtuzumabin renal transplantation. PMID: 20349607
7. Review article on CD52 structure and function. PMID: 11860230
8. HE5(CD52) mRNA and protein, expressed in epithelial cells of the distal epididymis, were not affected by the obstruction of the vas deferens. PMID: 14662784
9. The relationship between this differential insertion and differences in glycosylation of rat and human CD52 is discussed. PMID: 16266689
10. CD52 is widely expressed on human mast cells (MCs) and Waldenstrom's Macroglobulinemia bone marrow lymphoplasmacytic cells and provide the preclinical rationale for the use of alemtuzumab in the treatment of WM and possibly other MC-related disorders. PMID: 16796779
11. In this study, we identified the antigen of 4C8 mAb as CD52. CD52 is a costimulatory molecule for induction of CD4-positive T cells. PMID: 16797237
12. In contrast with CLL, the variable expression of CD52 among other hematologic malignancies suggests that target validation on a case-by-case basis will likely be necessary to guide the rational analysis of CAMPATH therapy. PMID: 17145843
13. Our data demonstrate differences in the intensity of the CD52 antigen expression between B-lymphocytes and tumor lymphocytes of B-CLL patients, and between B-CLL and SLL tumor cells. CD52 antigen is expressed at low level on CD34(+) cells. PMID: 17428002
14. The semenogelin-CD52 soluble form is a direct consequence of the liquefaction process in human semen. PMID: 17624925
15. We first showed the expression of CD52 in human cumulus cells. CD52 has some functional roles around fertilization in females as well as in males. PMID: 18647288
16. A review on CD52 expression and function PMID: 11257744
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亞細胞定位：

Cell membrane; Lipid-anchor, GPI-anchor.
數(shù)據(jù)庫鏈接：

HGNC: 1804

OMIM: 114280

KEGG: hsa:1043

STRING: 9606.ENSP00000363330

UniGene: Hs.276770