Recombinant Clostridium botulinum Botulinum neurotoxin type B (botB), partial

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中文名稱：

Recombinant Clostridium botulinum Botulinum neurotoxin type B(botB),partial
貨號(hào)：

CSB-EP318083CLQ
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP318083CLQ could indicate that this peptide derived from E.coli-expressed Clostridium botulinum botB.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP318083CLQ could indicate that this peptide derived from E.coli-expressed Clostridium botulinum botB.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

botB
Uniprot No.：

P10844
別名：

botBBotulinum neurotoxin type B; BoNT/B; Bontoxilysin-B) [Cleaved into: Botulinum neurotoxin B light chain; LC; EC 3.4.24.69); Botulinum neurotoxin B heavy chain; HC)]
種屬：

Clostridium botulinum
蛋白長(zhǎng)度：

Partial
來(lái)源：

E.coli
分子量：

63.3 kDa
表達(dá)區(qū)域：

1-427aa
氨基酸序列

MPVTINNFNYNDPIDNNNIIMMEPPFARGTGRYYKAFKITDRIWIIPERYTFGYKPEDFNKSSGIFNRDVCEYYDPDYLNTNDKKNIFLQTMIKLFNRIKSKPLGEKLLEMIINGIPYLGDRRVPLEEFNTNIASVTVNKLISNPGEVERKKGIFANLIIFGPGPVLNENETIDIGIQNHFASREGFGGIMQMKFCPEYVSVFNNVQENKGASIFNRRGYFSDPALILMHELIHVLHGLYGIKVDDLPIVPNEKKFFMQSTDAIQAEELYTFGGQDPSIITPSTDKSIYDKVLQNFRGIVDRLNKVLVCISDPNININIYKNKFKDKYKFVEDSEGKYSIDVESFDKLYKSLMFGFTETNIAENYKIKTRASYFSDSLPPVKIKNLLDNEIYTIEEGFNISDKDMEKEYRGQNKAINKQAYEEISKE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-B2M-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer，50% glycerol
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

The sequence encoding the 1-427aa of the Clostridium botulinum Botulinum neurotoxin type B (botB) was expressed in the E.coli cells. Each product has fused a 6xHis-B2M-tag at the N-terminus. The recombinant truncated Clostridium botulinum botB protein was purified by SDS-PAGE and got a purity of over 85%. The botB protein migrated to a molecular weight of about 64 kDa on the SDS-PAGE gel. And it was also validated by the LC-MS/MS analysis. In-stock botB proteins are offered now. This recombinant botB protein may find uses as an immunogen for specific antibody synthesis or in the studies of neurological diseases.

BotB is a Clostridium botulinum-expressed toxin that reduces muscle hyperactivity through its function at the neuromuscular junction. Once released into the cytosol, botB undergoes a multistep cascade of binding, internalization, cytosolic escape, and cleavage of the vesicle protein VAMP/SNAP-25 through its light chain (LV), resulting in the blockade of synaptic vesicle exocytosis and acetylcholine release at the neuromuscular junction. This process leads to transient denervation and weakening of muscle contractions responsible for excessive involuntary movements. The major neuronal receptor for botB is synaptotagmin II (Syt II). BotB is commonly used as a biopharmaceutics for neurological diseases.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin B which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol. Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release. Binds to host peripheral neuronal presynaptic membranes via synaptotagmins 1 and 2 (SYT1 and SYT2). Toxin binds to the membrane proximal extra-cytoplasmic region of host SYT1 and SYT2 that is transiently exposed outside of cells during exocytosis; exogenous gangliosides enhance binding and subsequent uptake of toxin into host cells. Toxin uptake into neural cells requires stimulation (incubation with K(+) to stimulate SYT protein receptor exposure); subsequently the toxin colocalizes with its receptor in host cells with a concomitant decrease in target protein (synaptobrevin-2/VAMP2) immunoreactivity. Toxin uptake can be blocked by the appropriate synaptotagmin protein fragments and gangliosides in cell culture and in mice. BoNT/B is a 'coincidence detector'; it requires simultaneous binding to coreceptor GT1b and low pH to transform into a membrane-bound, oligomeric channel. Whole toxin only has protease activity after reduction which releases LC.; Has proteolytic activity. After translocation into the eukaryotic host cytosol, inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-|-Phe-77' bond of synaptobrevin-2/VAMP2, blocking neurotransmitter release. In vitro the LC only has protease activity after reduction.; Responsible for host epithelial cell transcytosis, host nerve cell targeting and translocation of light chain (LC) into host cytosol. Composed of 3 subdomains; the translocation domain (TD), and N-terminus and C-terminus of the receptor-binding domain (RBD). The N-terminus of the TD wraps an extended belt around the perimeter of the LC; it does not seem to protect the active site, but might prevent premature LC dissociation from the translocation channel and protect toxin prior to translocation. Has 2 coreceptors; complex gangliosides found primarily on neural tissue and host synaptotagmin-1 and -2 (SYT1 and SYT2) which bind simultaneously to adjacent but separate sites at the tip of the HC. HC alone partially prevents uptake of whole toxin by neural cells, and delays paralysis onset by 160%. Binding probably positions the TD for integration into the synaptic vesicle membrane. The HC forms channels at low pH that mediate transport of the light chain (LC) from the endocytic vesicle to the cytosol. Binds gangliosides GD1b and GT1b. Gangliosides are not only a coreceptor, but also required for uptake into nerve cells. HC alone binds to host receptor proteins SYT1 and SYT2. Interaction with SYT1 protein does not require SYT1 glycosylation. The HC C-terminus (approximately residues 1079-1291) interacts with host SYT2. Has higher affinity for SYT2 than SYT1. Significantly decreases uptake and toxicity of whole BoNT/B and BoNT/G.
亞細(xì)胞定位：

[Botulinum neurotoxin type B]: Secreted. Host cell junction, host synapse, host presynaptic cell membrane.; [Botulinum neurotoxin B light chain]: Secreted. Host cytoplasm, host cytosol.; [Botulinum neurotoxin B heavy chain]: Secreted. Host cell junction, host synapse, host presynaptic cell membrane. Host cytoplasmic vesicle, host secretory vesicle, host synaptic vesicle membrane; Multi-pass membrane protein.
蛋白家族：

Peptidase M27 family