botB Antibody, HRP conjugated

^{Rare Species}

說明書

中文名稱：

botB兔多克隆抗體, HRP偶聯
貨號：

CSB-PA318083LB01CLQ
規格：

￥880
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Clostridium botulinum botB Polyclonal antibody
Uniprot No.：

P10844
基因名：

botB
別名：

botBBotulinum neurotoxin type B antibody; BoNT/B antibody; Bontoxilysin-B) [Cleaved into: Botulinum neurotoxin B light chain antibody; LC antibody; EC 3.4.24.69); Botulinum neurotoxin B heavy chain antibody; HC)] antibody
宿主：

Rabbit
反應種屬：

Clostridium botulinum
免疫原：

Recombinant Clostridium botulinum Botulinum neurotoxin type B protein (1-427AA)
免疫原種屬：

Clostridium botulinum
標記方式：

HRP
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA
Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin B which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol. Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release. Binds to host peripheral neuronal presynaptic membranes via synaptotagmins 1 and 2 (SYT1 and SYT2). Toxin binds to the membrane proximal extra-cytoplasmic region of host SYT1 and SYT2 that is transiently exposed outside of cells during exocytosis; exogenous gangliosides enhance binding and subsequent uptake of toxin into host cells. Toxin uptake into neural cells requires stimulation (incubation with K(+) to stimulate SYT protein receptor exposure); subsequently the toxin colocalizes with its receptor in host cells with a concomitant decrease in target protein (synaptobrevin-2/VAMP2) immunoreactivity. Toxin uptake can be blocked by the appropriate synaptotagmin protein fragments and gangliosides in cell culture and in mice. BoNT/B is a 'coincidence detector'; it requires simultaneous binding to coreceptor GT1b and low pH to transform into a membrane-bound, oligomeric channel. Whole toxin only has protease activity after reduction which releases LC.; Has proteolytic activity. After translocation into the eukaryotic host cytosol, inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-|-Phe-77' bond of synaptobrevin-2/VAMP2, blocking neurotransmitter release. In vitro the LC only has protease activity after reduction.; Responsible for host epithelial cell transcytosis, host nerve cell targeting and translocation of light chain (LC) into host cytosol. Composed of 3 subdomains; the translocation domain (TD), and N-terminus and C-terminus of the receptor-binding domain (RBD). The N-terminus of the TD wraps an extended belt around the perimeter of the LC; it does not seem to protect the active site, but might prevent premature LC dissociation from the translocation channel and protect toxin prior to translocation. Has 2 coreceptors; complex gangliosides found primarily on neural tissue and host synaptotagmin-1 and -2 (SYT1 and SYT2) which bind simultaneously to adjacent but separate sites at the tip of the HC. HC alone partially prevents uptake of whole toxin by neural cells, and delays paralysis onset by 160%. Binding probably positions the TD for integration into the synaptic vesicle membrane. The HC forms channels at low pH that mediate transport of the light chain (LC) from the endocytic vesicle to the cytosol. Binds gangliosides GD1b and GT1b. Gangliosides are not only a coreceptor, but also required for uptake into nerve cells. HC alone binds to host receptor proteins SYT1 and SYT2. Interaction with SYT1 protein does not require SYT1 glycosylation. The HC C-terminus (approximately residues 1079-1291) interacts with host SYT2. Has higher affinity for SYT2 than SYT1. Significantly decreases uptake and toxicity of whole BoNT/B and BoNT/G.
亞細胞定位：

[Botulinum neurotoxin type B]: Secreted. Host cell junction, host synapse, host presynaptic cell membrane.; [Botulinum neurotoxin B light chain]: Secreted. Host cytoplasm, host cytosol.; [Botulinum neurotoxin B heavy chain]: Secreted. Host cell junction, host synapse, host presynaptic cell membrane. Host cytoplasmic vesicle, host secretory vesicle, host synaptic vesicle membrane; Multi-pass membrane protein.
蛋白家族：

Peptidase M27 family

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