Phospho-SMAD5 (S463/S465) Recombinant Monoclonal Antibody
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中文名稱：磷酸化-SMAD5 (S463/S465)重組抗體
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貨號：CSB-RA859108A465phHU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in Hela whole cell lysate
All lanes Phospho-SMAD5 antibody at 0.835μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 KDa
Observed band size: 52 KDa
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其他：
產品詳情
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產品描述：Phospho-SMAD5 (S463/S465) Recombinant Monoclonal Antibody（CSB-RA859108A465phHU）是針對SMAD5蛋白磷酸化修飾位點S463/S465開發的高特異性重組單克隆抗體。SMAD5作為轉化生長因子-β（TGF-β）信號通路的關鍵介質分子，其Ser463/Ser465位點的磷酸化是激活下游信號傳遞的重要標志，參與調控細胞分化、增殖及組織發育等生物學過程。本抗體通過重組表達技術制備，具有批次間一致性高、交叉反應性低的特點，經嚴格驗證可特異性識別磷酸化修飾的SMAD5蛋白，適用于ELISA及Western Blot（WB）等實驗場景。在WB檢測中推薦使用1:500-1:5000稀釋度，能夠清晰檢測目標蛋白條帶，助力研究人員探究TGF-β/BMP信號通路的分子機制、干細胞命運調控或胚胎發育相關研究。該產品可為蛋白質翻譯后修飾研究、信號轉導通路分析及疾病模型構建提供可靠工具，適用于基礎科研領域的體外細胞實驗或組織樣本檢測。
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Uniprot No.：
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基因名：
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別名：DKFZp781C1895 antibody; DKFZp781O1323 antibody; Dwfc antibody; hSmad5 antibody; JV5 1 antibody; JV5-1 antibody; MAD homolog 5 antibody; MAD, mothers against decapentaplegic homolog 5 antibody; MADH 5 antibody; MADH5 antibody; Mothers against decapentaplegic homolog 5 antibody; mothers against decapentaplegic, drosophila, homolog of, 5 antibody; Mothers against DPP homolog 5 antibody; MusMLP antibody; SMA and MAD related protein 5 antibody; SMAD 5 antibody; SMAD family member 5 antibody; SMAD, mothers against DPP homolog 5 antibody; Smad5 antibody; SMAD5_HUMAN antibody
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反應種屬：Human
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免疫原：A synthesized peptide derived from Human Phospho-SMAD5 (S463/S465)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：3A9
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
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基因功能參考文獻：
- We demonstrated high expression of miR-145 associated with late stage and unfavorable prognosis of esophageal cancer. We identified SMAD5 as direct target of miR-145, the suppressed expression of which consequently led to increased cell proliferation and migration/invasion. PMID: 29852786
- Smad5 acts as a intracellular pH messenger and maintains the bioenergetic homeostasis of cells by regulating cytoplasmic metabolic machinery. PMID: 28675158
- Data suggest that differences in expression levels in granulosa-like tumor cells and granulosa cells (GCs) from patients with polycystic ovary syndrome (PCOS) are due to increased expression of microRNA-27a-3p in GCs caused by insulin resistance in PCOS; microRNA-27a-3 expression is up-regulated in GCs in PCOS; overexpression of miR-27a-3p inhibits SMAD5 expression and promotes apoptosis. (SMAD5 = SMAD family member 5) PMID: 29029022
- Here the involvement of the pathway in adult brain function is suggested. This exploratory study establishes a strategy to better identify neuronal molecular signatures that are potentially associated with mental illness and cognitive deficits. We propose that the SMAD pathway may be a novel target in addressing cognitive deficit of SZ in future studies. PMID: 27727244
- miR-23a and miR-27a target SMAD5 and regulate apoptosis in human granulosa cells via the FasL-Fas pathway PMID: 26400397
- Our findings suggest that suppression of miR-222-3p activity promoted osteogenic differentiation hBMSCs through regulating Smad5-RUNX2 signaling axis. PMID: 26809090
- The polycomb group protein L3MBTL1 represses a SMAD5-mediated hematopoietic transcriptional program in human pluripotent stem cells. PMID: 25754204
- among the 15 SNPs, rs3206634 was significantly associated with KD in a recessive model (odds ratio = 2.31, p = 0.019), whereas there was no association between any of the 15 SNPs and CALs. PMID: 24163009
- Specific gene siRNAs knock-down further confirmed the osteogenic effects of Genistein on BMP2, SMAD5 and RUNX2 protein expression PMID: 24339730
- ATP production by NaF promotes hypertrophy-like changes via activation of phospho-Smad5 PMID: 23384547
- found that restoration of SMAD5, in addition to the TGF-beta type II receptor, which was epigenetically silenced by the latent viral protein latency-associated nuclear antigen, sensitized BC3 cells to the cytostatic effect of TGF-beta signaling PMID: 22013049
- Studies indicate SMAD5beta isoform plays role in hematopoietic stem cell homeostasis. PMID: 20148926
- regulatory signals are active at transcriptionally subnuclear sites PMID: 12060751
- Smad5 activation by BMP4 in human hematopoietic cells results in significantly increased proliferation of erythroid progenitors and formation of glycophorin-A+ cells. PMID: 12064918
- up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection PMID: 12473652
- Results demonstrate a mitochondrial distribution of Smad5 in non-stimulated chondroprogenitor cells. PMID: 12849988
- SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma PMID: 14670176
- In mature human B cells, BMP-6 inhibited cell growth, and rapidly induced phosphorylation of Smad5. PMID: 15877825
- We used homology-modeling techniques to generate a reliable molecular model of the Smad5 MH1 domain based on the crystal structure of Smad3 MH1 domain PMID: 16243555
- SMAD5 gene was found to be associated with schizophrenia. PMID: 18298822
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亞細胞定位：Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
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蛋白家族：Dwarfin/SMAD family
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組織特異性：Ubiquitous.
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數據庫鏈接：
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