Phospho-SMAD5 (S463+S465) Recombinant Monoclonal Antibody

說明書

中文名稱：

磷酸化-SMAD5 (S463+S465)重組抗體
貨號(hào)：

CSB-RA859108A463phHU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA859108A463phHU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

Phospho-SMAD5 (S463+S465) Recombinant Monoclonal Antibody（CSB-RA859108A463phHU）是一款特異性識(shí)別SMAD5蛋白雙磷酸化位點(diǎn)（S463和S465）的高靈敏度抗體，適用于研究TGF-β超家族信號(hào)通路的調(diào)控機(jī)制。SMAD5作為TGF-β/BMP信號(hào)轉(zhuǎn)導(dǎo)的關(guān)鍵介質(zhì)，其磷酸化狀態(tài)直接參與調(diào)控細(xì)胞分化、胚胎發(fā)育及組織穩(wěn)態(tài)等生物學(xué)過程。該抗體通過重組單克隆技術(shù)制備，具有高批次穩(wěn)定性和特異性，經(jīng)嚴(yán)格驗(yàn)證可應(yīng)用于ELISA和免疫組化（IHC）實(shí)驗(yàn)，其中IHC推薦使用1:50-1:200稀釋比例，能夠清晰檢測石蠟包埋組織切片中目標(biāo)蛋白的磷酸化修飾水平。其在信號(hào)通路激活分析、疾病模型研究（如腫瘤微環(huán)境調(diào)控、纖維化疾病或骨骼發(fā)育異常機(jī)制）等科研領(lǐng)域具有重要應(yīng)用價(jià)值，為探索SMAD5依賴的細(xì)胞信號(hào)網(wǎng)絡(luò)及其病理生理功能提供可靠工具。本產(chǎn)品僅限科學(xué)研究使用。
Uniprot No.：

Q99717
基因名：

SMAD5
別名：

DKFZp781C1895 antibody; DKFZp781O1323 antibody; Dwfc antibody; hSmad5 antibody; JV5 1 antibody; JV5-1 antibody; MAD homolog 5 antibody; MAD, mothers against decapentaplegic homolog 5 antibody; MADH 5 antibody; MADH5 antibody; Mothers against decapentaplegic homolog 5 antibody; mothers against decapentaplegic, drosophila, homolog of, 5 antibody; Mothers against DPP homolog 5 antibody; MusMLP antibody; SMA and MAD related protein 5 antibody; SMAD 5 antibody; SMAD family member 5 antibody; SMAD, mothers against DPP homolog 5 antibody; Smad5 antibody; SMAD5_HUMAN antibody
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from Human Phospho-SMAD5 (S463+S465)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

2G11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
基因功能參考文獻(xiàn)：
1. We demonstrated high expression of miR-145 associated with late stage and unfavorable prognosis of esophageal cancer. We identified SMAD5 as direct target of miR-145, the suppressed expression of which consequently led to increased cell proliferation and migration/invasion. PMID: 29852786
2. Smad5 acts as a intracellular pH messenger and maintains the bioenergetic homeostasis of cells by regulating cytoplasmic metabolic machinery. PMID: 28675158
3. Data suggest that differences in expression levels in granulosa-like tumor cells and granulosa cells (GCs) from patients with polycystic ovary syndrome (PCOS) are due to increased expression of microRNA-27a-3p in GCs caused by insulin resistance in PCOS; microRNA-27a-3 expression is up-regulated in GCs in PCOS; overexpression of miR-27a-3p inhibits SMAD5 expression and promotes apoptosis. (SMAD5 = SMAD family member 5) PMID: 29029022
4. Here the involvement of the pathway in adult brain function is suggested. This exploratory study establishes a strategy to better identify neuronal molecular signatures that are potentially associated with mental illness and cognitive deficits. We propose that the SMAD pathway may be a novel target in addressing cognitive deficit of SZ in future studies. PMID: 27727244
5. miR-23a and miR-27a target SMAD5 and regulate apoptosis in human granulosa cells via the FasL-Fas pathway PMID: 26400397
6. Our findings suggest that suppression of miR-222-3p activity promoted osteogenic differentiation hBMSCs through regulating Smad5-RUNX2 signaling axis. PMID: 26809090
7. The polycomb group protein L3MBTL1 represses a SMAD5-mediated hematopoietic transcriptional program in human pluripotent stem cells. PMID: 25754204
8. among the 15 SNPs, rs3206634 was significantly associated with KD in a recessive model (odds ratio = 2.31, p = 0.019), whereas there was no association between any of the 15 SNPs and CALs. PMID: 24163009
9. Specific gene siRNAs knock-down further confirmed the osteogenic effects of Genistein on BMP2, SMAD5 and RUNX2 protein expression PMID: 24339730
10. ATP production by NaF promotes hypertrophy-like changes via activation of phospho-Smad5 PMID: 23384547
11. found that restoration of SMAD5, in addition to the TGF-beta type II receptor, which was epigenetically silenced by the latent viral protein latency-associated nuclear antigen, sensitized BC3 cells to the cytostatic effect of TGF-beta signaling PMID: 22013049
12. Studies indicate SMAD5beta isoform plays role in hematopoietic stem cell homeostasis. PMID: 20148926
13. regulatory signals are active at transcriptionally subnuclear sites PMID: 12060751
14. Smad5 activation by BMP4 in human hematopoietic cells results in significantly increased proliferation of erythroid progenitors and formation of glycophorin-A+ cells. PMID: 12064918
15. up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection PMID: 12473652
16. Results demonstrate a mitochondrial distribution of Smad5 in non-stimulated chondroprogenitor cells. PMID: 12849988
17. SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma PMID: 14670176
18. In mature human B cells, BMP-6 inhibited cell growth, and rapidly induced phosphorylation of Smad5. PMID: 15877825
19. We used homology-modeling techniques to generate a reliable molecular model of the Smad5 MH1 domain based on the crystal structure of Smad3 MH1 domain PMID: 16243555
20. SMAD5 gene was found to be associated with schizophrenia. PMID: 18298822
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亞細(xì)胞定位：

Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
蛋白家族：

Dwarfin/SMAD family
組織特異性：

Ubiquitous.
數(shù)據(jù)庫鏈接：

HGNC: 6771

OMIM: 603110

KEGG: hsa:4090

STRING: 9606.ENSP00000441954

UniGene: Hs.167700

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