PGAM1 Recombinant Monoclonal Antibody

說明書

中文名稱：

PGAM1重組抗體
貨號：

CSB-RA935337A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate
All lanes: PGAM1 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 29 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA935337A0HU PGAM1重組單克隆抗體是針對磷酸甘油酸變位酶1（PGAM1）開發的高特異性科研試劑。該靶標作為糖酵解通路關鍵酶，在細胞能量代謝和生物合成過程中發揮重要作用，其表達異常與多種病理過程密切相關。本產品采用重組技術制備，經嚴格驗證可在Western Blot實驗中穩定識別天然及重組PGAM1蛋白，檢測結果呈現清晰單一條帶，推薦使用稀釋范圍為1:500至1:5000，同時適用于ELISA平臺檢測。其高靈敏度和低背景特性使其成為研究腫瘤代謝重編程、細胞增殖調控以及氧化應激機制的有效工具，特別適用于體外培養細胞模型的蛋白表達分析、代謝通路研究及相關小分子抑制劑的藥效評價等基礎科研領域。該抗體具備優異的批次間穩定性，為科研工作者探索PGAM1在疾病發生發展中的分子機制提供可靠保障。
Uniprot No.：

P18669
基因名：

PGAM1
別名：

Phosphoglycerate mutase 1 (EC 5.4.2.11) (EC 5.4.2.4) (BPG-dependent PGAM 1) (Phosphoglycerate mutase isozyme B) (PGAM-B), PGAM1, PGAMA
反應種屬：

Human, Mouse, Rat
免疫原：

A synthesized peptide derived from human PGAM1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

9A5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.
基因功能參考文獻：
1. High PGAM1 expression is associated with Pancreatic Ductal Adenocarcinoma Metastasis. PMID: 29386088
2. conformation and dynamics of the C-terminal region in human phosphoglycerate mutase 1 PMID: 28748916
3. When BPGM is knocked out, 1,3-BPG can directly phosphorylate PGAM1. In this case, PGAM1 phosphorylation and activity are decreased, but nevertheless sufficient to maintain normal glycolytic flux and cellular growth rate. PMID: 28805803
4. study provided the first evidence revealing a non-metabolic function of PGAM1 in promoting cell migration, and gained new insights into the role of PGAM1 in cancer progression. PMID: 27991922
5. Data show that tyrosine 26 phosphorylation enhances the binding of phosphoglycerate mutase 1 (PGAM1) to its substrates through generating electrostatic environment and structural features that are advantageous to the binding. PMID: 28076845
6. PGAM1 in promoting homologous recombination repair in tumor cell lines. Suggest potential therapeutic opportunity for PGAM1 inhibitors in combination with PARP inhibitors in cancer treatment. PMID: 28122957
7. PGAM1 may be associated with the grade of glioma and be involved in the biological behavior of glioma cells. PGAM1 might be a novel therapeutic target in glioma. PMID: 27572934
8. PGAM1 correlates with spermatogenic dysfunction and affects the function of cell proliferation, apoptosis and migration. PMID: 25701843
9. Our finding showed that PGAM1 might serve as a promising therapeutic target for UBC. PMID: 26655504
10. PGAM1 is highly expressed in clear cell renal cell carcinoma and correlated with clinicalpathological features, which may contribute to tumor formation and progression. PMID: 26464696
11. PGAM is acetylated at lysines 100/106/113/138 in its central region, and a member of the Sirtuin family (class III deacetylase), SIRT2, is responsible for its deacetylation. PMID: 25195573
12. Tyrosine26 phosphorylation represents an additional acute mechanism underlying phosphoglycerate mutase 1 upregulation. PMID: 23653202
13. Phosphoglycerate mutase 1 (PGAM1) contributes to biosynthesis regulation by controlling intracellular levels of its substrate, 3-phosphoglycerate (3-PG), and product, 2-phosphoglycerate (2-PG). PMID: 23153533
14. PGAM1 deacetylation and activity are directly controlled by Sirt1. PMID: 22157007
15. histidine-phosphorylated PGAM1 correlated with expression of PKM2 in cancer cell lines; decreased pyruvate kinase activity in PKM2-expressing cells allows PEP-dependent histidine phosphorylation of PGAM1 and may provide an alternate glycolytic pathway PMID: 20847263
16. Our studies suggested that PGAM1 plays an important role in hepatocarcinogenesis PMID: 20403181
17. Results identified phosphoglycerate mutase 1 that showed elevated levels of protein carbonyls in inferior parietal lobule (IPL) from subjects with early stage-Alzheimer's disease. PMID: 19686046
18. Cloning, purification, crystallization and preliminary crystallographic analysis of human phosphoglycerate mutase PMID: 15388943
19. Phosphoglycerate mutase may have a role in preventing non-spherocytic anemia [case report] PMID: 15710582
20. We report two patients in whom phosphoglycerate mutase (PGAM) deficiency was associated with the triad of exercise-induced cramps, recurrent myoglobinuria, and tubular aggregates in the muscle biopsy. PMID: 16881065
顯示更多

收起更多
蛋白家族：

Phosphoglycerate mutase family, BPG-dependent PGAM subfamily
組織特異性：

Expressed in the liver and brain. Not found in the muscle.
數據庫鏈接：

HGNC: 8888

OMIM: 172250

KEGG: hsa:5223

STRING: 9606.ENSP00000359991

UniGene: Hs.632918

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
XRCC5 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
XDH Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WT1 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WFDC2 Recombinant Monoclonal Antibody

Applications: ELISA, WB, FC

Species Reactivity: Human