N Recombinant Monoclonal Antibody

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說(shuō)明書

中文名稱：

N重組抗體
貨號(hào)：

CSB-RA33255A1GMY
規(guī)格：

￥1540
圖片：
Western Blot
Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY)
SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY)
Secondary
Peroxidase-Affinipure Goat Anti-Human IgG Fc Fragment Specific at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa

The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC₅₀ is 1.586 ng/ml.

In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A1GMY) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml), and the sensitivity is very good.

ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A1GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.

The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC₅₀ of N Recombinant Antibody.

The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC₅₀ of N Recombinant Antibody.

The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC₅₀ of N Recombinant Antibody.
其他：

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產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA33255A1GMY N重組單克隆抗體是針對(duì)病毒核衣殼蛋白（N蛋白）研發(fā)的高特異性科研試劑，適用于病毒學(xué)基礎(chǔ)研究與體外檢測(cè)分析。該抗體采用基因重組技術(shù)制備，可精準(zhǔn)識(shí)別N蛋白保守結(jié)構(gòu)域，在ELISA、Western Blot及膠體金免疫層析（GICA）三大檢測(cè)平臺(tái)中均表現(xiàn)出優(yōu)異性能。經(jīng)多體系驗(yàn)證顯示，其ELISA檢測(cè)效價(jià)高達(dá)1:50000，Western Blot在1:500-1:5000稀釋范圍內(nèi)能清晰呈現(xiàn)目標(biāo)條帶，GICA應(yīng)用時(shí)推薦1:1000-1:10000梯度檢測(cè)，展現(xiàn)良好的線性響應(yīng)與批次穩(wěn)定性。該產(chǎn)品已通過抗原表位特異性驗(yàn)證，對(duì)常見冠狀病毒N蛋白無(wú)交叉反應(yīng)，適用于病毒復(fù)制機(jī)制研究、重組蛋白表達(dá)驗(yàn)證、快速檢測(cè)試紙條開發(fā)等科研場(chǎng)景，為病原體檢測(cè)技術(shù)開發(fā)及抗病毒藥物篩選提供可靠工具。本產(chǎn)品嚴(yán)格遵循質(zhì)量管理體系生產(chǎn)，每批次均提供完整質(zhì)檢報(bào)告，確保實(shí)驗(yàn)數(shù)據(jù)的可重復(fù)性。
Uniprot No.：

P0DTC9
別名：

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
反應(yīng)種屬：

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
免疫原：

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)
免疫原種屬：

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Mouse scFv fusion with human IgG1 Fc
純化方式：

Affinity-chromatography
克隆號(hào)：

1A6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, GICA

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:10000-1:50000
WB	1:500-1:5000
GICA	1:1000-1:10000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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靶點(diǎn)詳情

功能：

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
亞細(xì)胞定位：

Virion. Host cytoplasm.
蛋白家族：

Betacoronavirus nucleocapsid protein family

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