SARS-CoV-2 N ELISA Kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

新型冠狀病毒N蛋白 (SARS-CoV-2 N) 酶聯(lián)免疫試劑盒（CSB-EL33251）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、樣本樣本中的SARS-CoV-2 N含量。SARS-CoV-2 N是新冠病毒的重要靶點。，新冠疫情的全球大流行促使對該病毒各靶點深入研究。研究機制主要聚焦于N蛋白在病毒復(fù)制、轉(zhuǎn)錄及包裝等過程中的作用，通過抑制該靶點有望開發(fā)出抗新冠病毒藥物。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，靈敏度為0.78 ng/mL。評估動物模型呼吸道拭子中的病毒載量；監(jiān)測疫苗免疫后體液抗體水平變化；用于病毒蛋白與宿主互作研究中的定量分析；建立病毒感染的動態(tài)監(jiān)測模型；用于抗病毒藥物篩選實驗中靶蛋白表達水平的定量評估；為新冠肺炎相關(guān)基礎(chǔ)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
縮寫：

SARS-CoV-2 N
Uniprot No.：

P0DTC9
樣本類型：

serum, plasma, swabs
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Infectious Diseases
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
亞細胞定位：

Virion. Host cytoplasm.
蛋白家族：

Betacoronavirus nucleocapsid protein family