PIN4 Recombinant Monoclonal Antibody

說明書

中文名稱：

PIN4重組抗體
貨號：

CSB-RA916699A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: U251 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate
All lanes: PIN4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14, 17, 15 kDa
Observed band size: 12-17 kDa

IHC image of CSB-RA916699A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of MCF-7 cell with CSB-RA916699A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 598-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
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靶點詳情

產品詳情

產品描述：

CSB-RA916699A0HU重組單克隆抗體是針對PIN4蛋白研發的高特異性試劑，適用于多種蛋白檢測場景。PIN4（Peptidyl-prolyl cis/trans isomerase）屬于肽酰脯氨酰順反異構酶家族，通過調控磷酸化蛋白的構象變化參與細胞周期調控、應激響應及腫瘤發生等過程，在癌癥與神經退行性疾病研究中備受關注。本抗體經ELISA、Western Blot（WB）、免疫組化（IHC）和免疫熒光（IF）多平臺驗證，在WB實驗中可清晰識別天然蛋白，IHC顯示組織定位特異性，IF檢測呈現亞細胞水平的高分辨率信號，推薦稀釋度分別為WB 1:500-1:2000、IHC 1:50-1:200、IF 1:50-1:200。其低交叉反應性和高批次穩定性使其成為研究PIN4在細胞周期異常、蛋白折疊障礙及相關疾病分子機制中的理想工具，適用于腫瘤生物學、神經病理學及基礎蛋白質相互作用研究領域。
Uniprot No.：

Q9Y237
基因名：

PIN4
別名：

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (EC 5.2.1.8) (Parvulin-14) (Par14) (hPar14) (Parvulin-17) (Par17) (hPar17) (Peptidyl-prolyl cis-trans isomerase Pin4) (PPIase Pin4) (Peptidyl-prolyl cis/trans isomerase EPVH) (hEPVH) (Rotamase Pin4), PIN4
反應種屬：

Human
免疫原：

A synthesized peptide derived from human PIN4
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

14A7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.; Isoform 2 binds to double-stranded DNA.
基因功能參考文獻：
1. Par14 can be described as an endogenous non-histone chromatin protein, which binds DNA in vivo PMID: 25645591
2. Ca(2+)/Calmodulin binding to the N-terminal of Par17 causes steric hindrance of the Par17 active site, thus interfering with the Par17/tubulin interaction. PMID: 25940090
3. Data indicate that Pin1 prolyl isomerase active site cysteine, Cys113, is highly susceptible to oxidation. PMID: 25595659
4. Data indicate the association of Par14 with insulin receptor substrate 1 (IRS-1) in human HepG2 cells overexpressing both as well as endogenously in the mouse liver. PMID: 23720771
5. The N-terminal basic domain of human parvulin hPar14 is responsible for the entry to the nucleus and high-affinity DNA-binding. PMID: 12144781
6. subcellular localization and DNA binding properties of hPar14 are regulated by phosphorylation of the N-terminal domain PMID: 12860119
7. Identification of a longer Parvulin isoform (Par17) that has an extension at the 5' end including a 75 bp extended open reading frame. PMID: 16522211
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亞細胞定位：

[Isoform 1]: Nucleus, nucleolus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Note=Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.; [Isoform 2]: Mitochondrion. Mitochondrion matrix. Note=Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide.
蛋白家族：

PpiC/parvulin rotamase family, PIN4 subfamily
組織特異性：

Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much m
數據庫鏈接：

HGNC: 8992

OMIM: 300252

KEGG: hsa:5303

STRING: 9606.ENSP00000362773

UniGene: Hs.655623

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