MTA2 Recombinant Monoclonal Antibody
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中文名稱：MTA2 Recombinant Monoclonal Antibody
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貨號：CSB-RA568219A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: Jurkat whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg)
All lanes: MTA2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 75 kDa
Observed band size: 70 kDa
Exposure time：3min -
IHC image of CSB-RA568219A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA568219A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Immunofluorescence staining of A431 cell with CSB-RA568219A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing jurkat cells stained with CSB-RA568219A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：MTA2
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別名：Metastasis-associated protein MTA2 (Metastasis-associated 1-like 1) (MTA1-L1 protein) (p53 target protein in deacetylase complex), MTA2, MTA1L1 PID
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反應種屬：Human, Mouse
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免疫原：A synthesized peptide from human MTA2 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：11D11
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins.
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基因功能參考文獻：
- This study is the first to demonstrate that SNHG5 is a critical and powerful regulator that is involved in GC progression through trapping MTA2 in the cytosol. PMID: 27065326
- Taken together with previous findings this suggests, that MTA-RBBP is a stable complex, with a central role in the initial assembly of the human NuRD complex. PMID: 28179136
- These results indicated there might be a tight correlation among MTA2, Ki-67 and hepatocellular carcinoma prognosis PMID: 26722504
- MTA2 is a crucial biomarker that is closely related with prognosis of colorectal carcinoma and also a potential molecular target for evaluating the prognosis and treatment of CRC. PMID: 26261611
- MTA2 overexpression enhances colony formation and tumor growth of gastric cancer cells, but not plays important role in cancer cell migration and metastasis. IL-11 is one of the downstream effectors of MTA2 in regulating gastric cancer cells growth PMID: 25929737
- MTA-2 protein may facilitate the invasive potential of non-small-cell lung cancer cells through the inhibition of the cell adhesion molecule Ep-CAM and E-cadherin, suggesting that it might be a potential therapeutic target in NSCLC. PMID: 25969565
- High MTA2 protein and mRNA expression is associated with glioma. PMID: 25048531
- MTA2 acts as a central hub for cytoskeletal organization and transcription and provides a link between nuclear and cytoskeletal organization. PMID: 25394532
- MTA2 depletion could significantly inhibit human breast cancer cell growth and metastasis, implying that MTA2 might be involved in the progression of breast cancer. PMID: 25081667
- Study demonstrates that hBD-3 inhibits the progression of colon cancer in a paracrine fashion through downregulation of MTA2 expression. PMID: 24969834
- Mta2 and Tipin cooperate to maintain replication fork integrity, especially on regions that are intrinsically difficult to duplicate. PMID: 24830473
- MTA2 knockdown impairs invasion and metastasis of gastric cancer cells. PMID: 24010737
- results reveal a novel post-translational regulation of MTA2 by the way of p300-dependent acetylation, which is important for tumor cells growth and migration and provides a potential target for clinical cancer research PMID: 24468085
- High expression levels of MTA2 is associated with pancreatic ductal adenocarcinoma. PMID: 23400716
- MTA2 is highly expressed in the primary lesions of gastric cancer than that in adjacent non-cancerous tissues, and is closely related with tumor invasion. MTA2 expression is elevated in Sp1 positive gastric cancer. PMID: 23158992
- Sertoli cell-specific expression of MTA2 is required for transcriptional regulation of FSHR gene during spermatogenesis. PMID: 23086931
- High nuclear MTA2 expression is associated with thymoma. PMID: 22833212
- Our data suggested that MTA2 might play roles in both the nucleus and cytoplasm in the progression of NSCLC. PMID: 22585429
- The expression of MTA2 had positive correlation with clinical stage and lymph node metastasis. PMID: 20704817
- interaction with mCpG-binding domain of MBD2 PMID: 12124384
- MTA2 is a repressor of estrogen receptor (ER)alpha activity and that it could represent a new therapeutic target of ERalpha action in human breast tumors PMID: 16645043
- Findings indicate a tight correlation between the MTA2 expression level and hepatocellular carcinoma size and differentiation. PMID: 19702911
- Deacetylation and functional interactions by the PID/MTA2-associated NuRD complex may represent an important pathway to regulate p53 function. PMID: 11099047
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亞細胞定位：Nucleus.
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組織特異性：Widely expressed.
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數據庫鏈接：
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