KIFC1 Recombinant Monoclonal Antibody

說明書

中文名稱：

KIFC1 Recombinant Monoclonal Antibody
貨號：

CSB-RA057545A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA057545A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing jurkat cells stained with CSB-RA057545A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
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靶點詳情

產品詳情

Uniprot No.：

Q9BW19
基因名：

KIFC1
別名：

Kinesin-like protein KIFC1 (Kinesin-like protein 2) (Kinesin-related protein HSET), KIFC1, HSET KNSL2
反應種屬：

Human
免疫原：

A synthesized peptide from human KIFC1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

1F9
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. Regulates cilium formation and structure.
基因功能參考文獻：
1. Data show that kinesin-related protein HSET (HSET) is incapable of forming asters from preformed, nongrowing microtubule (non-MT) MTs, but rapidly forms MT asters in the presence of soluble (non-MT) tubulin. PMID: 29985404
2. KIFC1 interacts with F508del-CFTR. PMID: 30066085
3. nuclear KIFC1 (nKIFC1) correlated significantly with Ki67 in White triple-negative breast cancer (TNBCs) but not in African American (AA) TNBCs, suggesting that nKIFC1 is not merely a surrogate for proliferation in AA TNBCs. PMID: 28218233
4. Results identified Stathmin as a direct target of KIFC1. PMID: 28885720
5. The overexpression of KIFC1 was correlated closely with the progression of hepatocellular carcinoma (HCC) and poor prognosis, and suggested that the expression levels of KIFC1 are a potential prognostic biomarker for HCC. PMID: 29620256
6. Circulating pri-miRNA-944 and 3662 can improve non-invasive potential non-small cell lung cancer detection of operable stages of SCC and AC PMID: 28964573
7. These findings reveal a new stationary binding model that kinesin-14 KIFC1 proteins function as crosslinkers between the Golgi apparatus and the microtubules and contribute to the central positioning and structural maintenance of the Golgi apparatus. PMID: 28430595
8. Taken together, these results suggest that up-regulation of KIFC1 may induce docetaxel resistance. PMID: 27665358
9. Low post translational modifications of KIFC1 protein is associated with neoplasms. PMID: 28209915
10. KIFC1 is overexpressed in GC. PMID: 27176706
11. Association of KIFC1 gene expression and centrosome amplification with high-grade serous ovarian carcinoma. PMID: 26992853
12. stabilization of the centrosome-spindle pole interface by the CEP215-HSET complex could promote survival of cancer cells containing supernumerary centrosomes. PMID: 26987684
13. KIFC1 silencing significantly reduces breast cancer cell viability. PMID: 26177331
14. Data provides the first evidence of centrosome clustering-independent activities of HSET that fuel tumor progression. PMID: 25788277
15. We show that some are new substrates of the anaphase-promoting complex/cyclosome and validate KIFC1 and RacGAP1/Cyk4 as two such targets involved respectively in timely mitotic spindle disassembly and cell spreading PMID: 24857844
16. Our data underscore KIFC1 as a putative biomarker that predicts worse prognosis, poor overall survival and may serve as a potential marker of onset of metastatic dissemination in ovarian cancer patients. PMID: 25028599
17. Study demonstrates microtubule tip-tracking of human kinesin-14 HSET and show that EB1 is sufficient to induce this dynamic microtubule end localization. PMID: 24039245
18. Acentrosomal spindle assembly mechanisms are hyperactive in cancer cells and promote HSET. PMID: 22946058
19. we suggest that KIFC1 plays an essential role for bipolar MTOC formation and maintaining chromosomal stability in the mitosis . PMID: 23318213
20. A case-control analysis was carried out by comparing the genotype distribution of six KIFC1 single-nucleotide polymorphisms between 93 aspirin exacerbated respiratory disease cases and 96 aspirin-tolerant asthma controls in a Korean population PMID: 22201025
21. The intense placental expression of KIFC1 in syncytiotrophoblast and KIF17 in vascular endothelium suggests that both proteins might be important in a cargo-transport system. KIFC1 and KIF17 expression are increased of both in preeclamptia and diabetes. PMID: 19679349
22. An analysis was made of the weak interactions of ADP-Unc104 and ADP-kinesin with microtubules and their inhibition by MAP2. PMID: 17326138
23. HSET has a role in controlling spindle length by cross-linking and sliding microtubules. PMID: 19116309
24. High KIFC1 expression is associated with metastatic spread to the brain in primary non-small cell lung cancer. PMID: 19190132
25. Chromosome congression in HSET + hNuf2 co-depleted cells required the plus-end directed motor CENP-E , which has been implicated in the gliding of mono-oriented kinetochores alongside adjacent K-fibres. PMID: 19525938
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亞細胞定位：

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Early endosome.
蛋白家族：

TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, NCD subfamily
數據庫鏈接：

HGNC: 6389

OMIM: 603763

KEGG: hsa:3833

STRING: 9606.ENSP00000393963

UniGene: Hs.436912

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