GSS Recombinant Monoclonal Antibody

說明書

中文名稱：

GSS Recombinant Monoclonal Antibody
貨號：

CSB-RA188887A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: HEK293 whole cell lysate(30μg), Hela whole cell lysate(30μg), PC-3 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), SH-SY5Y whole cell lysate(30μg), A431 whole cell lysate(30μg), Mouse liver tissue lysate(30μg), Rat liver tissue lysate(30μg), Mouse lung tissue lysate(30μg)
All lanes: GSS antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time：2min

Immunofluorescence staining of A549 cell with CSB-RA188887A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing A431 cells stained with CSB-RA188887A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

P48637
基因名：

GSS
別名：

Glutathione synthetase (GSH synthetase) (GSH-S) (EC 6.3.2.3) (Glutathione synthase), GSS
反應種屬：

Human, Mouse, Rat
免疫原：

A synthesized peptide from human GSS protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

2H9
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner. Glutathione (gamma-glutamylcysteinylglycine, GSH) is the most abundant intracellular thiol in living aerobic cells and is required for numerous processes including the protection of cells against oxidative damage, amino acid transport, the detoxification of foreign compounds, the maintenance of protein sulfhydryl groups in a reduced state and acts as a cofactor for a number of enzymes.
基因功能參考文獻：
1. Data suggest that the potential of clusterin and glutathione synthetase (GSH-S) as platelet biomarkers for early detection of colorectal cancer (CRC) could improve existing screening modalities in clinical application. PMID: 28849249
2. Mutation in Glutathione Synthase gene is associated with chronic metabolic acidosis in glutathione synthetase deficiency. PMID: 26669244
3. Four SNPs (rs7265992, rs6060124, rs7260770, and rs4911455) in GSS were significantly associated with bladder cancer recurrence after transurethral resection and BCG treatment. PMID: 25851338
4. In this study, clinical, biochemical, and genetic aspects of five Chinese 5-oxoprolinuria patients with OPLAH or GSS gene mutations were investigated. PMID: 25851806
5. Studied the role of protein-protein interactions in the structural stability, activity and allostery of enzymes using the obligate homodimer human glutathione synthetase as an ideal model. PMID: 25070563
6. The findings indicate that Asp458 is essential for hGS catalysis and that it impacts the allostery of hGS. PMID: 21771585
7. These results imply that residues V44 and V45 are integral to the stability of human glutathione synthetase. PMID: 21683691
8. We have shown that susceptibility to health effects of air pollution on lung function growth is associated with genetic variation in the GSS gene PMID: 20802163
9. Single-nucleotide polymorphism in glutathione synthetase is associated with small-cell lung cancer. PMID: 20439344
10. This research indicates that Gly369 and Gly370 have essential roles in hGS, while Gly371 has a lesser involvement. PMID: 20800579
11. the cause of cellular ATP depletion in nephrotic cystinosis may be the futile cycle, formed between two ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine synthetase and 5-oxoprolinase PMID: 20413906
12. A novel alternative splicing variant (ASV) of the GSS gene was identified in 10 human normal tissues and five human cancer cell lines. PMID: 19672693
13. Glutathione synthetase deficiency: is gamma-glutamylcysteine accumulation a way to cope with oxidative stress in cells with insufficient levels of glutathione? PMID: 12638941
14. analysis of conserved residues of human glutathione synthetase PMID: 14990577
15. The cloning and characterization of a 2.2 kb 5'-flanking region of the human glutathione synthetase gene is reported. PMID: 15890065
16. glutathione synthetase autosomal mutations result in glutathione synthetase deficiency, which may cause progressive retinal dystrophy with hyperpigmentations and maculopathy [case report] PMID: 17206463
17. Glutathione synthase expression may indicate better survival in early stage adenocarcinoma of the lung, and manipulation of glutathione synthase may be a potential basis for treatment of some non-small cell lung cancers. PMID: 17234469
18. Severe glutathione synthetase deficiency is associated with progressive retinal dystrophy of the rod-cone type, affecting the central retina with advanced macular edema in adulthood. PMID: 19111905
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相關疾病：

Glutathione synthetase deficiency (GSS deficiency); Glutathione synthetase deficiency of erythrocytes (GLUSYNDE)
蛋白家族：

Eukaryotic GSH synthase family
數據庫鏈接：

HGNC: 4624

OMIM: 231900

KEGG: hsa:2937

STRING: 9606.ENSP00000216951

UniGene: Hs.82327

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