CD58 Recombinant Monoclonal Antibody

說明書

中文名稱：

CD58重組抗體
貨號：

CSB-RA166177A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Raji whole cell lysate, K562 whole cell lysate, Hela whole cell lysate
All lanes: CD58 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29, 27, 26 kDa
Observed band size: 55-70 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CD58重組單克隆抗體（產品編號：CSB-RA166177A0HU）是針對CD58抗原研發的高特異性抗體。CD58（又稱LFA-3）是一種免疫調節分子，主要通過與T細胞表面CD2受體結合，參與T細胞活化及免疫突觸形成，在抗感染免疫和自身免疫性疾病中發揮關鍵作用。本產品經ELISA和Western Blot（WB）嚴格驗證，可特異性識別天然及重組CD58蛋白，在WB實驗中推薦使用1:500-1:2000稀釋比例，實驗結果顯示清晰的靶蛋白檢測條帶，且背景信號低，重復性優異。該抗體適用于體外研究CD58在免疫細胞激活、腫瘤免疫微環境調控以及感染性疾病模型中的表達與功能機制，可作為探索T細胞介導的免疫應答、免疫耐受及相關信號通路的有效工具。科研人員可通過該產品進一步解析CD58與CD2互作對免疫調控的影響，為開發免疫相關疾病的干預策略提供實驗依據。本產品僅限于科學研究使用。
Uniprot No.：

P19256
基因名：

CD58
別名：

Lymphocyte function-associated antigen 3 (Ag3) (Surface glycoprotein LFA-3) (CD antigen CD58), CD58, LFA3
反應種屬：

Human
免疫原：

A synthesized peptide derived from human CD58
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

13G5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.
基因功能參考文獻：
1. indicate that including both CD58 and CD81 markers in addition to CD19, CD34, CD20, CD38, and CD10 are helpful in minimal residual disease detection by flow cytometry PMID: 29500862
2. Mutations and copy number loss of CD58 or TP53 are identified to be an independent negative prognostic factors for diffuse large B cell lymphoma. PMID: 27825110
3. A new neuromyelitis optica spectrum disorders susceptibility variant was identified, rs56302466, on the CD58 gene, in a Han Chinese population. PMID: 28601281
4. data suggest that loss of CD58 is a potential immune escape mechanism of HL tumor cells, especially in clinically aggressive disease PMID: 27467287
5. Cytometry analysis evidenced a specific expression profile on reticulocytes of SCA infants, with notably an increased expression of the adhesion molecules Lu/BCAM, ICAM-4 and LFA-3, both in percentage of positive cells and in surface density. PMID: 26137540
6. Frequent inactivating mutations of CD58 in classical Hodgkin lymphoma cell lines, but their rare occurrence in primary Hodgkin and Reed/Sternberg cells. PMID: 26194173
7. SNPs in CD58 are associated with increased risk of candidemia. PMID: 25197941
8. This study demonstrated that the Polymorphism, Single Nucleotide of CD58 is associated with multiple sclerosis in man in Russia. PMID: 25903733
9. Results indicate that CD58 is a novel cell-surface marker that functionally regulates self-renewal of Colorectal tumor-initiating cells. PMID: 24727892
10. Linkage disequilibrium show evidence that mir-548ac rs1414273 variant is strongly associated with Multiple sclerosis (MS)-associated haplotype and confirms the single nucleotide polymorphisms within the first intron of CD58 been related too. [review] PMID: 25795118
11. The positive correlation is established between content of polymorphic nuclear monocytes and level of expression of molecules of LFA-1, ICAM-1, LFA-3, and PECAM-1. PMID: 25884075
12. Genetic variations in CD58 were associated with the susceptibility of neuromyelitis optica in a Korean population. PMID: 24655566
13. In 21 percent of diffuse large B cell lymphoma cases, lesions involve the CD58 gene, which encodes a molecule involved in T and natural killer cell-mediated responses PMID: 22137796
14. Two large cohorts of systemic sclerosis (SSc) patients of European Caucasian ancestry do not support the implication of ITGAM, ITGAX, and CD58 genes in the genetic susceptibility of SSc, although they were identified as autoimmune disease risk genes. PMID: 21362770
15. Seven selected CD58 single-nucleotide polymorphisms were found to not affect aspirin-exacerbated respiratory disease susceptibility in a Korean population. PMID: 21726122
16. Studies indicate that SNP in IL7RA, IL2RA, CD58 and CLEC16A genes has been consistently associated with MS. PMID: 20450971
17. Data show that coculture with activated T cells upregulated expression of CD54 and CD58 and secretion of galectin-1 by MSCs. PMID: 20570633
18. Studies indicate that five SNPs showed genome-wide significant association with MS: HLA-DRA, IL7R, IL2RA, CD58 and CLEC16A. PMID: 19834503
19. Signal-dependent adhesion of resting NK cells initiated by expression of ICAM-1 is greatly enhanced by coexpression of LFA-3, even in the absence of cytokines. PMID: 12496412
20. The complement inhibitor CD59 and the lymphocyte function-associated antigen-3 (LFA-3, CD58) genes possess functional binding sites for the p53 tumor suppressor protein. PMID: 12553064
21. Transmembrane CD58 may trigger signaling independently of the GPI-linked isoform. PMID: 15093607
22. Human cells transformed with Ad12 demonstrated reduced expression of cell surface LFA-3. PMID: 15963548
23. The level of CD58 molecule (in both serum and PBMC form) of patients with hepatitis B is related to the degree of liver damage. PMID: 16830383
24. Susceptibility gene for multiple sclerosis in Australians. PMID: 18650830
25. We found variable, but persistently elevated levels of sLFA-3 throughout the various phases and types of the hemorrhagic fever with renal syndrome, which suggest that sLFA-3 levels have correlation with disease stages. PMID: 18820826
26. Cross-linking of CD58 induces protein tyrosine phosphorylation of BLNK, Syk and PLCgamma, and activation of ERK and Akt/PKB. PMID: 19268704
27. Study reports additional genetic and transcriptomic evidence for the role of CD58 (LFA-3) in multiple sclerosis (MS) susceptibility using a Swedish case-control material, with a result that closely mimics that of De Jager et al. PMID: 19497873
28. Genetic variants at CD58, is associated with rheumatoid arthritis risk PMID: 19898481
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亞細胞定位：

[Isoform 1]: Cell membrane; Single-pass type I membrane protein.
數據庫鏈接：

HGNC: 1688

OMIM: 153420

KEGG: hsa:965

STRING: 9606.ENSP00000358501

UniGene: Hs.34341

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