CBX2 Recombinant Monoclonal Antibody

說明書

中文名稱：

CBX2重組抗體
貨號：

CSB-RA632623A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: U251 whole cell lysate, Ntera-2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, Rat heart tissue
All lanes: CBX2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57, 24 kDa
Observed band size: 60-72 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA632623A0HU重組單克隆抗體是針對表觀遺傳調控核心因子CBX2開發的高特異性科研工具。該抗體靶向Polycomb抑制復合物（PRC1）的關鍵成員CBX2蛋白，該蛋白通過介導組蛋白H3K27me3修飾參與基因沉默、干細胞維持及細胞命運決定等重要生物學過程，其功能異常與胚胎發育異常及腫瘤發生密切相關。經嚴格驗證，本抗體在Western Blot實驗中可清晰識別內源性CBX2蛋白表達，推薦使用濃度為1:500至1:2000；同時具備ELISA檢測應用兼容性，為研究者提供多重實驗選擇。該產品適用于探索多能性維持、細胞重編程、腫瘤表觀遺傳失調等研究領域，尤其適用于染色質動態調控機制解析、基因沉默模型構建及靶向PRC1復合物的功能研究。通過高批間一致性和低交叉反應性的優勢，該抗體可為表觀遺傳學、發育生物學和腫瘤微環境研究提供可靠支持，所有應用均嚴格限定于基礎科研范疇。
Uniprot No.：

Q14781
基因名：

CBX2
別名：

Chromobox protein homolog 2, CBX2
反應種屬：

Human, Rat
免疫原：

A synthesized peptide derived from human CBX2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

24A2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) or at 'Lys-27' (H3K27me3). Plays a role in the lineage differentiation of the germ layers in embryonic development. Involved in sexual development, acting as activator of NR5A1 expression.
基因功能參考文獻：
1. study indicates a distinct function of the shorter form of CBX2 and by identifying several of its unique targets, can advance our understanding of DSD pathogenesis and ultimately DSD diagnosis and management. PMID: 29998616
2. These results suggest that CBX2's phosphorylation is critical for its transcriptional repression of target genes. PMID: 28992316
3. Data show that basal-like subgroup was enriched for aggressive tumors and somatic mutations in trithorax-group genes and it overexpressed polycomb genes EZH2 and CBX2. PMID: 26431491
4. up-regulated in metastatic castration-resistant prostate cancer and elevated expression correlates with poor clinical outcome PMID: 26877821
5. CBX2 role in the sex development cascade is to stimulate the male pathway and concurrently inhibit the female pathway. PMID: 25569159
6. CBX2 upregulation and amplification significantly correlated with metastatic progression and lower overall survival in many cancer types, particularly those of the breast PMID: 25225902
7. This study does not support CBX2 gene disruption as a common cause of gonadal gonadal disorders of sex development. PMID: 23219007
8. Two distinct mechanisms are involved in CBX2-mediated gene silencing. The short CBX2-2 isoform would repress the transcription in a PRC1-independent fashion, whereas gene repression by the long CBX2-1 isoform is mediated by the PRC1 protein complex. PMID: 22419124
9. CDYL functions as a molecular bridge between PRC2 and the repressive chromatin mark H3K27me3, forming a positive feedback loop to facilitate the establishment and propagation of H3K27me3 modifications along the chromatin PMID: 22009739
10. hPc2 serves as a SUMO E3 ligase for cystathionine beta-synthase, increasing the efficiency of sumoylation. PMID: 19107218
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相關疾病：

46,XY sex reversal 5 (SRXY5)
亞細胞定位：

Nucleus. Chromosome.
數據庫鏈接：

HGNC: 1552

OMIM: 602770

KEGG: hsa:84733

STRING: 9606.ENSP00000308750

UniGene: Hs.368410

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