Phospho-TBC1D4 (T642) Antibody

文獻引用 (2) 說明書

中文名稱：

磷酸化-TBC1D4 (T642)兔多克隆抗體
貨號：

CSB-PA080083
規格：

￥1090
圖片：
Western blot analysis of L929 using p-TBC1D4 (T642) antibody.
其他：

產品詳情
產品評價
引用文獻
相關產品
靶點詳情

產品詳情

Uniprot No.：

O60343
基因名：

TBC1D4
別名：

Acrg embryonic lethality (mouse) minimal region ortholog antibody; Acrg embryonic lethality minimal region ortholog antibody; Acrg embryonic lethality mouse minimal region ortholog antibody; Akt substrate of 160 kDa antibody; AS 160 antibody; AS160 antibody; BUB2 antibody; CDC16 antibody; KIAA0603 antibody; NIDDM5 antibody; TBC (Tre 2 BUB2 CDC16) domain containing protein antibody; TBC Tre 2 BUB2 CDC16 domain containing protein antibody; TBC1 D4 antibody; TBC1 domain family member 4 antibody; Tbc1d4 antibody; TBCD4_HUMAN antibody; Tre-2 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Synthesized peptide derived from Human TBC1D4 around the phosphorylation site of T642.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, IHC, IF, ELISA

推薦稀釋比：

Application	Recommended Dilution
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:5000

Protocols：

Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻

LRRK2 negatively regulates glucose tolerance via regulation of membrane translocation of GLUT4 in adipocytes F Kawakami,FEBS open bio,2023
Leucine-rich repeat kinase 2 negatively regulates glucose tolerance via regulation of membrane translocation of Glucose transporter type 4 in adipocytes F Kawakami,researchsquare,2022

產品評價

靶點詳情

功能：

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
基因功能參考文獻：
1. C-terminal region (CTR) region is largely alpha-helical and mediates TBC1D4 RabGAP dimerization PMID: 28545963
2. Disruption of TBC1D4 is common among North American Inuit, resulting in exclusively elevated postprandial glucose. This leads to underdiagnosis of type 2 diabetes, unless an OGTT is performed. PMID: 27561922
3. AS160 regulates glucose-independent eukaryotic cell proliferation through p21-dependent control of the cell cycle. PMID: 27152871
4. AS160 and TBC1D1 phosphorylations were evident 30 min after exercise. PMID: 24876356
5. homozygous carriers of a nonsense p.Arg684Ter variant have markedly higher concentrations of plasma glucose and serum insulin 2 hours after an oral glucose load compared with individuals with other genotypes PMID: 25043022
6. Findings suggest that a dampening of insulin-induced phosphorylation of AS160 on specific sites in skeletal muscle contributes to the insulin resistance evident in a sedentary aging population PMID: 23801578
7. insulin resistance in muscles from healthy individuals is associated with suppression of site-specific phosphorylation of AS160 PMID: 22028408
8. AS160 interacts with the large cytoplasmic NP domain of the alpha-subunit of the Na(+),K(+)-ATPase. AMP-stimulated protein kinase (AMPK) and AS160 participate in a common pathway to modulate the cell surface expression of the Na(+),K(+)-ATPase. PMID: 20943949
9. Crystal structures of human TBC1D1 and TBC1D4 (AS160) RabGTPase-activating protein (RabGAP) domains reveal critical elements for GLUT4 translocation. PMID: 21454505
10. AS160 phosphotyrosine-binding domain constructs inhibit insulin-stimulated GLUT4 vesicle fusion with the plasma membrane PMID: 21454690
11. Impaired insulin-induced site-specific TBC1D4 phosphorylation may contribute to skeletal muscle insulin resistance in type 2 diabetes. PMID: 20938636
12. results show AS160 phosphorylation level is frequently increased in breast cancer; results implicate a possible role of AS160 in breast tumorigenesis and suggest that p-AS160 might be useful as a marker and a potential novel treatment target PMID: 20574165
13. WNK1 promotes cell surface expression of glucose transporter GLUT1 by regulating a Tre-2/USP6-BUB2-Cdc16 domain family member 4 (TBC1D4)-Rab8A complex PMID: 20937822
14. S711 is a novel TBC1D4 phosphorylation site regulated by AMPK in skeletal muscle. PMID: 19923418
15. KIAA0603 is likely to be a Rab GAP that participates in the regulation of activated T cells in atopic dermatitis PMID: 15304337
16. In this study, we determined the expression of and in vivo insulin action on AS160 in skeletal muscle of normal and type 2 diabetic patients. PMID: 15919790
17. results indicate that AS160 is a Rab GAP, and suggest novel Rabs that may participate in GLUT4 translocation PMID: 15971998
18. AS160 is phosphorylated in a time-dependent manner during moderate-intensity exercise. PMID: 17077344
19. Regulation of AS160 and interaction with 14-3-3 in skeletal muscle are influenced by resistance exercise and insulin but do not fully explain the effect of resistance exercise on whole-body insulin action. PMID: 17369524
20. Effects of endurance exercise training on insulin signaling and AS160 in human skeletal muscle PMID: 17513702
21. AS160 is a common target of insulin, IGF-1, EGF, PMA and AICAR, these stimuli induce distinctive patterns of phosphorylation and 14-3-3 binding, mediated by at least four protein kinases. PMID: 17617058
22. Impaired insulin signaling through Akt and AS160 in part explains insulin resistance at the molecular level in skeletal muscle in polycystic ovary syndrome. PMID: 17977950
23. AS160, previously recognized as a key player in insulin signaling in skeletal muscle and adipose tissue, is also a major effector of protein kinase B/Akt signaling in the beta-cell. PMID: 18276765
24. The transcript variant 2 of AS160 in contrast to full-length AS160 is a novel regulator of glucose transport that positively influences glucose-uptake rates. PMID: 18771725
25. muscle TBC1D4 phosphorylation across the leg is increased during recovery following resistance exercise PMID: 18845784
26. Specific phosphorylation of TBC1D4 in human skeletal muscle in response to physiological exercise-induced hyperinsulinemia. PMID: 19252894
27. Prematurely truncated TBC1D4 protein tended to increase basal cell membrane GLUT4 levels (P = 0.053) and significantly reduced insulin-stimulated GLUT4 cell membrane translocation (P < 0.05). PMID: 19470471
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相關疾病：

Diabetes mellitus, non-insulin-dependent, 5 (NIDDM5)
亞細胞定位：

Cytoplasm. Note=Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
組織特異性：

Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland,
數據庫鏈接：

HGNC: 19165

OMIM: 612465

KEGG: hsa:9882

STRING: 9606.ENSP00000366863

UniGene: Hs.210891

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