Phospho-BLNK (Tyr96) Antibody

說明書

中文名稱：

磷酸化-BLNK (Tyr96)兔多克隆抗體
貨號：

CSB-PA598542
規格：

￥2454
圖片：
Western blot analysis of extracts from COLO205 tissue using BLNK (Phospho-Tyr96) antibody.The lane on the right is treated with the antigen-specific peptide.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) BLNK Polyclonal antibody
Uniprot No.：

Q8WV28
基因名：

BLNK
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Peptide sequence around phosphorylation site of tyrosine 96(D-S-Y(p)-E-P) derived from Human BLNK .
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
純化方式：

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
濃度：

It differs from different batches. Please contact us to confirm it.
產品提供形式：

Liquid
應用范圍：

ELISA,WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
基因功能參考文獻：
1. vesicular signaling scaffolds are required for B cell activation indicates that vesicles may deliver preassembled signaling cargo to sites of BCR activation. PMID: 25140054
2. early Ca(2+) fluxing provides feed-forward signal amplification by promoting anchoring of the PLCgamma2 C2 domain to phospho-SLP65. PMID: 24166973
3. up-regulation of BLNK is associated with RUNX1 mutations in cytogenetically normal acute myeloid leukemia. PMID: 22689681
4. Live cell imaging and co-immunoprecipitation experiments confirmed that both SLP65 and CIN85 are both required for the onset and progression phases of B-cell antigen receptor signal transduction. PMID: 21822214
5. 16 of the 34 childhood pre-B acute lymphoblastic leukaemia samples that were tested showed a complete loss or drastic reduction of SLP-65 expression PMID: 12761551
6. The BLNK protein is present in the majority of mediastinal B cell lymphomas. PMID: 15744341
7. In B cells SLP-65 exists in a 180 kDa complex as well as in monomeric form. PMID: 16356554
8. V(H) gene rearrangement represents a frequent feature in B-lymphoid malignancy, which can be attributed to SLP65 deficiency in many cases. PMID: 16636677
9. Syk is required to link phosphorylated SLP-65 to Ca(2+) mobilization. PMID: 17681949
10. Plasmacytoid dendritic cells express a signalosome consisting of Lyn, Syk, Btk, Slp65 (Blnk) and PLCgamma2. Triggering CD303 leads to tyrosine phosphorylation of Syk, Slp65, PLCgamma2 & cytoskeletal proteins. PMID: 18022864
11. BLNK prevents aneuploidy by inhibiting cytokinesis. PMID: 19018766
12. BLNK recruits active H-Ras to the BCR complex, which is essential for sustained surface expression of BCR in the form of the cap and for the signal leading to functional ERK activation PMID: 19218240
13. Data show that SLP-65 phosphorylation acts upstream for signal initiation and also downstream during selective processing of the B cell receptor signal. PMID: 19372136
14. Identifies BLNK as a potential target for the protein-tyrosine phosphatase SHP-1. PMID: 15588985
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相關疾病：

Agammaglobulinemia 4, autosomal recessive (AGM4)
亞細胞定位：

Cytoplasm. Cell membrane. Note=BCR activation results in the translocation to membrane fraction.
組織特異性：

Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
數據庫鏈接：

HGNC: 14211

OMIM: 604515

KEGG: hsa:29760

STRING: 9606.ENSP00000224337

UniGene: Hs.665244

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