BLNK Antibody

中文名稱：

BLNK兔多克隆抗體
貨號：

CSB-PA840984LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes: BLNK antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51, 49, 45 kDa
Observed band size: 51 kDa

IHC image of CSB-PA840984LA01HU diluted at 1:800 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescent analysis of Hela cells using CSB-PA840984LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) BLNK Polyclonal antibody
Uniprot No.：

Q8WV28
基因名：

BLNK
別名：

AGM4 antibody; B cell adapter containing SH2 domain protein antibody; B cell adapter containing Src homology 2 domain protein antibody; B cell adaptor containing SH2 domain antibody; B cell linker antibody; B cell linker protein antibody; B cell-specific adaptor protein antibody; B-cell activation antibody; B-cell adapter containing a SH2 domain protein antibody; B-cell adapter containing a Src homology 2 domain protein antibody; B-cell linker protein antibody; BASH antibody; Bca antibody; BLNK antibody; BLNK s antibody; BLNK_HUMAN antibody; Cytoplasmic adapter protein antibody; Ly 57 antibody; Ly57 antibody; Lymphocyte antigen 57 antibody; Lyw 57 antibody; Lyw57 antibody; MGC111051 antibody; SH2 domain-containing leukocyte protein; 65-KD antibody; SLP 65 antibody; SLP-65 antibody; SLP65 antibody; Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody; Src homology 2 domain containing leukocyte protein of 65 kDa antibody; Src homology 2 domain-containing leukocyte protein of 65 kDa antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human B-cell linker protein (41-337AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，BLNK Antibody (CSB-PA840984LA01HU)，的標記方式是Non-conjugated。對于BLNK Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA840984LB01HU	BLNK Antibody, HRP conjugated	ELISA
FITC	CSB-PA840984LC01HU	BLNK Antibody, FITC conjugated
Biotin	CSB-PA840984LD01HU	BLNK Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
基因功能參考文獻：
1. vesicular signaling scaffolds are required for B cell activation indicates that vesicles may deliver preassembled signaling cargo to sites of BCR activation. PMID: 25140054
2. early Ca(2+) fluxing provides feed-forward signal amplification by promoting anchoring of the PLCgamma2 C2 domain to phospho-SLP65. PMID: 24166973
3. up-regulation of BLNK is associated with RUNX1 mutations in cytogenetically normal acute myeloid leukemia. PMID: 22689681
4. Live cell imaging and co-immunoprecipitation experiments confirmed that both SLP65 and CIN85 are both required for the onset and progression phases of B-cell antigen receptor signal transduction. PMID: 21822214
5. 16 of the 34 childhood pre-B acute lymphoblastic leukaemia samples that were tested showed a complete loss or drastic reduction of SLP-65 expression PMID: 12761551
6. The BLNK protein is present in the majority of mediastinal B cell lymphomas. PMID: 15744341
7. In B cells SLP-65 exists in a 180 kDa complex as well as in monomeric form. PMID: 16356554
8. V(H) gene rearrangement represents a frequent feature in B-lymphoid malignancy, which can be attributed to SLP65 deficiency in many cases. PMID: 16636677
9. Syk is required to link phosphorylated SLP-65 to Ca(2+) mobilization. PMID: 17681949
10. Plasmacytoid dendritic cells express a signalosome consisting of Lyn, Syk, Btk, Slp65 (Blnk) and PLCgamma2. Triggering CD303 leads to tyrosine phosphorylation of Syk, Slp65, PLCgamma2 & cytoskeletal proteins. PMID: 18022864
11. BLNK prevents aneuploidy by inhibiting cytokinesis. PMID: 19018766
12. BLNK recruits active H-Ras to the BCR complex, which is essential for sustained surface expression of BCR in the form of the cap and for the signal leading to functional ERK activation PMID: 19218240
13. Data show that SLP-65 phosphorylation acts upstream for signal initiation and also downstream during selective processing of the B cell receptor signal. PMID: 19372136
14. Identifies BLNK as a potential target for the protein-tyrosine phosphatase SHP-1. PMID: 15588985
顯示更多

收起更多
相關疾?。?/div>
Agammaglobulinemia 4, autosomal recessive (AGM4)
亞細胞定位：

Cytoplasm. Cell membrane. Note=BCR activation results in the translocation to membrane fraction.
組織特異性：

Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
數據庫鏈接：

HGNC: 14211

OMIM: 604515

KEGG: hsa:29760

STRING: 9606.ENSP00000224337

UniGene: Hs.665244

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
XRCC5 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
XDH Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WT1 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WFDC2 Recombinant Monoclonal Antibody

Applications: ELISA, WB, FC

Species Reactivity: Human