PNCK Antibody

中文名稱：

PNCK兔多克隆抗體
貨號：

CSB-PA754178LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Mouse brain tissue, Mouse kidney tissue
All lanes: PNCK antibody at 3.1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 41, 28, 47 kDa
Observed band size: 39 kDa

IHC image of CSB-PA754178LA01HU diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產品詳情
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相關產品
靶點詳情

產品詳情

產品描述：

The PNCK polyclonal antibody is a rabbit-derived antibody that has been specifically designed to target the PNCK protein. The immunogen used to generate this antibody is a recombinant human PNCK protein (1-115aa). The PNCK protein mainly acts as a serine/threonine kinase that plays a role in various cellular processes, including cell proliferation, migration, survival, and differentiation. PNCK overexpression has been detected in a variety of tumor types, including breast, ovarian, and prostate cancer, and can promote cancer cell proliferation, migration, and invasion.

This PNCK antibody has been purified using protein G affinity chromatography and has a purity of >95%. It has been validated for use in various applications, including ELISA, WB, and IHC. And it shows broad reactivity with human, mouse, and rat samples.
產品名稱：

Rabbit anti-Homo sapiens (Human) PNCK Polyclonal antibody
Uniprot No.：

Q6P2M8
基因名：

PNCK
別名：

Calcium/calmodulin dependent protein kinase type 1B antibody; Calcium/calmodulin-dependent protein kinase type 1B antibody; CaM KI beta antibody; CaM kinase I beta antibody; CaM kinase IB antibody; CaM-KI beta antibody; CaMK1b antibody; CaMKI beta antibody; CaMKI-beta antibody; EC 2.7.11.17 antibody; KCC1B_HUMAN antibody; MGC45419 antibody; PNCK antibody; Pregnancy up regulated non ubiquitously expressed CaM kinase antibody; Pregnancy up-regulated non-ubiquitously-expressed CaM kinase antibody
宿主：

Rabbit
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human Calcium/calmodulin-dependent protein kinase type 1B protein (1-115AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，PNCK Antibody (CSB-PA754178LA01HU)，的標記方式是Non-conjugated。對于PNCK Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA754178LB01HU	PNCK Antibody, HRP conjugated	ELISA
FITC	CSB-PA754178LC01HU	PNCK Antibody, FITC conjugated
Biotin	CSB-PA754178LD01HU	PNCK Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1.
基因功能參考文獻：
1. Pnck may be a marker of Trastuzumab resistance and possibly a therapeutic target in breast cancer. PMID: 25773930
2. the relationship between PNCK and prognosis in clear cell renal cell carcinoma PMID: 23634203
3. Pnck induces epidermal growth factor receptor degradation, most likely through perturbation of Hsp90 chaperone activity due to Hsp90 phosphorylation. Epidermal growth factor receptor degradation is coupled to proteasomal degradation of Pnck. PMID: 21325639
4. Pnck induces ligand-independent EGFR degradation, and it may represent an attractive therapeutic target in EGFR-regulated oncogenesis. PMID: 18562482
亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
數據庫鏈接：

HGNC: 13415

OMIM: 300680

KEGG: hsa:139728

STRING: 9606.ENSP00000405950

UniGene: Hs.436667

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