NTAN1 Antibody

中文名稱：

NTAN1兔多克隆抗體
貨號：

CSB-PA822176LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA822176LA01HU diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of U251 cells with CSB-PA822176LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) NTAN1 Polyclonal antibody
Uniprot No.：

Q96AB6
基因名：

NTAN1
別名：

NTAN1 antibody; Protein N-terminal asparagine amidohydrolase antibody; EC 3.5.1.121 antibody; Protein NH2-terminal asparagine amidohydrolase antibody; PNAA antibody; Protein NH2-terminal asparagine deamidase antibody; PNAD antibody; Protein N-terminal Asn amidase antibody; Protein N-terminal asparagine amidase antibody; Protein NTN-amidase antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Protein N-terminal asparagine amidohydrolase protein (219-310AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，NTAN1 Antibody (CSB-PA822176LA01HU)，的標記方式是Non-conjugated。對于NTAN1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA822176LB01HU	NTAN1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA822176LC01HU	NTAN1 Antibody, FITC conjugated
Biotin	CSB-PA822176LD01HU	NTAN1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

N-terminal asparagine deamidase that mediates deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by NTAN1/PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N-terminal peptidyl Asn.
基因功能參考文獻：
1. Results indicate that hNTAN1 is highly selective for the hydrolysis of N-terminal peptidyl L-Asn. PMID: 21375249
亞細胞定位：

Cytoplasm.
數據庫鏈接：

HGNC: 29909

OMIM: 615367

KEGG: hsa:123803

STRING: 9606.ENSP00000287706

UniGene: Hs.592045

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