EIF3K Antibody

文獻引用 (1) 說明書

中文名稱：

EIF3K兔多克隆抗體
貨號：

CSB-PA866204ESR1HU
規格：

￥440
圖片：
Western blot
All lanes: EIF3K antibody at 1.72µg/ml
Lane 1: THP-1 whole cell lysate
Lane 2: HT29 whole cell lysate
Lane 3: 293T whole cell lysate
Lane 4: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 26, 25 kDa
Observed band size: 26 kDa

Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA866204ESR1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA866204ESR1HU at dilution of 1:100

Immunoprecipitating EIF3K in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA866204ESR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA866204ESR1HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
其他：

產品詳情
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引用文獻
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) EIF3K Polyclonal antibody
Uniprot No.：

Q9UBQ5
基因名：

EIF3K
別名：

ARG134 antibody; eIF 3 p25 antibody; eIF 3 p28 antibody; eIF-3 p25 antibody; eIF-3 p28 antibody; EIF3-p28 antibody; eif3k antibody; EIF3K_HUMAN antibody; EIF3S12 antibody; Eukaryotic translation initiation factor 3 subunit 12 antibody; Eukaryotic translation initiation factor 3 subunit K antibody; HSPC029 antibody; M9 antibody; MSTP001 antibody; Muscle specific gene M9 protein antibody; Muscle-specific gene M9 protein antibody; PLAC 24 antibody; PLAC-24 antibody; PLAC24 antibody; PRO1474 antibody; PTD001 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Eukaryotic translation initiation factor 3 subunit K protein (1-218AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻

The non-coding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumour cells EP Booy,J Biol Chem,2020

產品評價

靶點詳情

功能：

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
基因功能參考文獻：
1. eIF3K subunit interacts directly with the PML protein. PMID: 24036361
2. identification of a novel 24-kDa protein that binds directly to dynein intermediate chain [PLAC-24] PMID: 12006665
3. eIF3k has three putative protein-binding surfaces and has potential RNA binding activity PMID: 15180986
4. cyclin D3 specifically interacted with eIF3k through its C-terminal domain; eIF3k distributed both in nucleus and cytoplasm and colocalized with cyclin D3 PMID: 15327989
5. eIF3k, originally identified as the smallest subunit of eukaryotic translation initiation factor 3 (eIF3) complexes, also localizes to keratin intermediate filaments and physically associates with K18 in epithelial cells. PMID: 18577580
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亞細胞定位：

Nucleus. Cytoplasm.
蛋白家族：

EIF-3 subunit K family
組織特異性：

Ubiquitous, with the highest levels of expression in brain, testis and kidney.
數據庫鏈接：

HGNC: 24656

OMIM: 609596

KEGG: hsa:27335

STRING: 9606.ENSP00000248342

UniGene: Hs.314359

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