DNAJA3 Antibody

中文名稱：

DNAJA3兔多克隆抗體
貨號：

CSB-PA836207LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, A549 whole cell lysate
All lanes: DNAJA3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53, 50, 34 kDa
Observed band size: 53 kDa

IHC image of CSB-PA836207LA01HU diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA836207LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of MCF-7 cells with CSB-PA836207LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating DNAJA3 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA836207LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA836207LA01HU (5µg) + Hela whole cell lysate (0.5mg)
Lane 3: Hela whole cell lysate (20µg)
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) DNAJA3 Polyclonal antibody
Uniprot No.：

Q96EY1
基因名：

DNAJA3
別名：

DNAJA3 antibody; HCA57 antibody; TID1DnaJ homolog subfamily A member 3 antibody; mitochondrial antibody; DnaJ protein Tid-1 antibody; hTid-1 antibody; Hepatocellular carcinoma-associated antigen 57 antibody; Tumorous imaginal discs protein Tid56 homolog antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human DnaJ homolog subfamily A member 3, mitochondrial protein (86-184AA )
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，DNAJA3 Antibody (CSB-PA836207LA01HU)，的標記方式是Non-conjugated。對于DNAJA3 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA836207LB01HU	DNAJA3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA836207LC01HU	DNAJA3 Antibody, FITC conjugated
Biotin	CSB-PA836207LD01HU	DNAJA3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.
基因功能參考文獻：
1. Impaired stoichiometry between mtHsp40 and mtHsp70 promotes Opa1L cleavage, leading to cristae opening, decreased OXPHOS, and triggering of mitochondrial fragmentation after reduction in their chaperone function. PMID: 25904328
2. The Role of the Phylogenetically Conserved Cochaperone Protein Droj2/DNAJA3 in NF-kappaB Signaling. PMID: 26245905
3. TID1 prevents complex I aggregation and support the existence of a TID1-mediated stress response to ATP synthase inhibition PMID: 24492964
4. Tid1 acts as a tumor suppressor by inhibiting EGFR signaling through interaction with EGFR/HSP70/HSP90 and enhancing EGFR ubiquitinylation and degradation. PMID: 23698466
5. Data show that elevated DnaJA3 induces dynamin-related protein 1 (Drp1)-depedendent mitochondrial fragmentation and decreased cell viability. PMID: 22595283
6. loss of hTid-1(S) expression in the basal layer of skin epidermis correlates with the enhanced HSP27 phosphorylation, keratinocyte hyperproliferation, and excess actin cytoskeleton organization in lesional psoriatic skin PMID: 22692211
7. Tid1 and CHIP play pivotal roles in affecting the levels of ErbB2 protein, and that both are significant prognostic indicators of breast cancer patient survival. PMID: 21710689
8. findings denote hTid-1(S) as an essential regulatory component of MetR signaling PMID: 21242965
9. hTid1 negatively regulates the expression and transcriptional activity of STAT5b and suppresses the growth of hematopoietic cells transformed by an oncogenic form of STAT5b. PMID: 21106534
10. htid is a tissue independent and evolutionarily conserved suppressor of ErbB-2. PMID: 20565727
11. amino-terminal segment of APC promotes cell sensitivity to apoptosis modulated through its binding to 40- and 43-kilodalton hTID-1 isoforms. PMID: 19900451
12. role as a repressor of Ikappa B kinase beta subunit PMID: 11927590
13. Tid-1S in Th2 cells following activation has a role in induction of apoptosis resistance during the activation of Th2 cells PMID: 12879007
14. an important cell death regulator and could exert tumor suppressor activity PMID: 15156195
15. findings suggest that the status of hTid-1 in gliomas may contribute to their susceptibility to cell death triggers PMID: 15589840
16. hTid-1 represses the activity of NF-kappaB through physical and functional interactions with the IKK complex and IkappaB PMID: 15601829
17. the carboxyl-terminal end of TID1 (residues 224-429) bound to Trk at the activation loop (Tyr(P)(683)-Tyr(684)(P)(684) PMID: 15753086
18. Tid1 is critical for the transition of double-negative to double-positive cells in early T cell development through modulation of antiapoptotic bcl-2 expression. PMID: 15879105
19. Tid1 negatively regulates the motility and metastasis of breast cancer cells, most likely through attenuation of nuclear factor kappaB activity on the promoter of the IL8 gene. PMID: 16204048
20. hTid1 may act as a chaperone to facilitate the folding, processing and maturation of Epstein-Barr virus-encoded BARF1 protein. PMID: 16518412
21. The association of Tid1 with chaperones and/or protein substrates in the cytosol provides a mechanism for the alternate fates and functions of Tid1 in mitochondrial and nonmitochondrial pathways. PMID: 16531398
22. The association of the endogenous Tid50/Tid48 proteins with the adenomatous polyposis coli (APC) tumor suppressor is shown. PMID: 17588722
23. The expression of the genes htid-1 and APC was altered in colorectal tumors. PMID: 18097612
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亞細胞定位：

Mitochondrion matrix. Cytoplasm, cytosol. Cell junction, synapse, postsynaptic cell membrane; Peripheral membrane protein.
組織特異性：

Widely expressed with highest levels in heart, liver, lung and skeletal muscles. Also expressed in keratinocytes.
數據庫鏈接：

HGNC: 11808

OMIM: 608382

KEGG: hsa:9093

STRING: 9606.ENSP00000262375

UniGene: Hs.459779

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