COTL1 Antibody

說明書

中文名稱：

COTL1兔多克隆抗體
貨號：

CSB-PA622751ESR1HU
規(guī)格：

￥440
圖片：
Western blot
All lanes: COTL1 antibody at 5μg/ml
Lane 1: Mouse brain tissue
Lane 2: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa

Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA622751ESR1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA622751ESR1HU at dilution of 1:100
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) COTL1 Polyclonal antibody
Uniprot No.：

Q14019
基因名：

COTL1
別名：

CLP antibody; Coactosin like 1 antibody; Coactosin-like protein antibody; COTL1 antibody; COTL1_HUMAN antibody; FLJ43657 antibody; MGC19733 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human, Mouse
免疫原：

Recombinant Human Coactosin-like protein (1-142AA)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis.
基因功能參考文獻(xiàn)：
1. Study uncovers a novel tumor-suppressor role for CLP/Cotl1 and identify the downstream effectors interleukin 24 (IL-24)/PERP and IL-24/MAPK/ERK/TGF-beta. PMID: 28925397
2. our combined results elucidated genetic and epigenetic silencing of miR-506-3p enhances COTL1 oncogene expression to foster NSCLC progression. PMID: 27893417
3. The roles of CLP and FLAP in in cellular leukotriene biosynthesis, were studied. PMID: 25034252
4. COTL1 regulates lamellipodia dynamics in part by protecting F-actin from cofilin-mediated disassembly. PMID: 24454796
5. PAI-RBP1 and C1orf142 expression levels are higher in 95D (high metastatic potential) than in 95C (low metastatic potential) non-small lung cancer cells, whereas COTL1 expression level is lower in 95D when compared to 95C cells. PMID: 22373659
6. Up-regulated COTL-1 expression in small cell lung cancer was validated by Western blot analysis, immunohistochemistry, and qRT-qPCR. PMID: 21047053
7. Results of the present study support a role for CLP as a chaperoning scaffold factor, influencing both the stability and the activity of 5-LO. PMID: 19807693
8. CLP 57-65 peptide elicits immediate-type hypersensitivity. Identical peptides are recognized by cellular and humoral immune systems to a tumor-associated antigen. CLP 15-24 and 104-113 might be used for peptide-based immunotherapy of HLA-A2(+) cancers. PMID: 11870627
9. NMR resonance assignments and the secondary structures of human Coactosin like protein (hCLP) D123N PMID: 15213466
10. coactosin-like protein (CLP) is an actin-binding protein as well as a 5-lipoxygenase binding partner PMID: 15333945
11. structural analysis shows that CLP forms a polymer along the crystallographic b axis with the exact same repeat distance as F-actin; a model for the CLP polymer and F-actin binding has therefore been proposed. PMID: 15459340
12. preliminary crystallographic studies of human coactosin-like protein (CLP) PMID: 15583396
13. Coactosin-like protein (CLP) up-regulates Ca(2+)-induced 5-lipoxygenase (5LO) activity, and increases the amount of Leukotriene A(4) formed by 5LO. PMID: 16924104
14. extended region of beta4-beta5 of hCLP (residue 66-75) was found very flexible and very important for F-actin binding. The C-terminal residues of human coactosin-like protein were not involved in F-actin binding PMID: 17070122
15. We found that coactosin-like1 (COTL1) were highly expressed in rheumatoid arthritis patients compared with healthy controls PMID: 19307756
顯示更多

收起更多
亞細(xì)胞定位：

Cytoplasm. Cytoplasm, cytoskeleton. Nucleus.
蛋白家族：

Actin-binding proteins ADF family, Coactosin subfamily
組織特異性：

Widely expressed with highest levels in placenta, lung, kidney and peripheral blood leukocytes and lower levels in brain, liver and pancreas.
數(shù)據(jù)庫鏈接：

HGNC: 18304

OMIM: 606748

KEGG: hsa:23406

STRING: 9606.ENSP00000262428

UniGene: Hs.289092

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
XRCC5 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
XDH Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WT1 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WFDC2 Recombinant Monoclonal Antibody

Applications: ELISA, WB, FC

Species Reactivity: Human