Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor protein (MANF)

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中文名稱：

人MANF重組蛋白
貨號(hào)：

CSB-AP002891HU
說明書：

MSDS Datasheet
規(guī)格：

￥852
圖片：
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>95% as determined by SDS-PAGE.
內(nèi)毒素：

Less than 1.0 EU/μg as determined by LAL method.
生物活性：

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of >50 IU/mg.
基因名：

MANF
Uniprot No.：

P55145
別名：

Arginine rich mutated in early stage tumors; Arginine rich protein; Arginine-rich protein; ARMET; ARMET protein; ARP; Manf; MANF_HUMAN; Mesencephalic astrocyte derived neurotrophic factor; Mesencephalic astrocyte-derived neurotrophic factor; MGC142148; MGC142150; Protein ARMET
種屬：

Homo sapiens (Human)
蛋白長(zhǎng)度：

Full Length of Mature Protein
來源：

E.Coli
分子量：

18.2 kDa
表達(dá)區(qū)域：

25-182aa
氨基酸序列

LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
蛋白標(biāo)簽：

Tag-Free
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

0.2 μm filtered PBS, pH 7.4 ,lyophilized
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

5-10 business days
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions.
基因功能參考文獻(xiàn)：
1. Our data indicate that increased MANF concentrations in serum are associated with the clinical manifestation of type 1 diabetes in children, but the exact mechanism behind the increase remains elusive PMID: 27356471
2. Sulfatide binding promotes cellular MANF uptake and cytoprotection from hypoxia-induced cell death. PMID: 29497057
3. Serum MANF level was higher in patients with newly diagnosed prediabetes and T2 Diabetes Mellitus than in normal glucose tolerance controls. MANF appears to be associated with Matsuda Index, QUICKI and HOMA-IR in prediabetes patients. PMID: 28216543
4. the role of two short sequence motifs within the carboxy-(C) terminal domain of MANF in its neuroprotective activity, was studied. PMID: 26720341
5. the selective expression of MANF in splenocytes may be involved in plasma cell differentiation and immune regulation. PMID: 26429332
6. MANF is a protein that interacts with RTN1-C PMID: 25543119
7. We demonstrate that Armet and Creld2 are genotype-specific ER stress response proteins with substrate specificities, and that aggregation of mutant matrilin-3 is a key disease trigger in MED that could be exploited as a potential therapeutic target PMID: 23956175
8. MANF binding to the plasma membrane also required the RTDL sequence and was inhibited with a peptide known to interact with KDELRs, suggesting MANF binds KDELRs at the surface. PMID: 23255601
9. MANF and C-MANF protect neurons intracellularly as efficiently as Ku70. PMID: 21047780
10. Pretreatment with adeno-associated-virus vector containing human MANF reduces the volume of cerebral infarction and facilitates behavioral recovery in experimental stroke rats. PMID: 20685313
11. Armet is a novel secreted mediator of the adaptive pathway of unfolded protein response. PMID: 18561914
12. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggest that it has important functions both under normal and pathological conditions in many tissue types. PMID: 18718866
13. structures of MANF and CDNF were solved; structure explains why MANF and CDNF are bifunctional; neurotrophic activity may reside in the N-terminal domain and ER stress response in the C-terminal domain PMID: 19258449
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亞細(xì)胞定位：

Secreted. Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen.
蛋白家族：

ARMET family
數(shù)據(jù)庫鏈接：

HGNC: 15461

OMIM: 601916

KEGG: hsa:7873

STRING: 9606.ENSP00000432799

UniGene: Hs.436446