Sheep tri-iodothyronine (T3) ELISA kit

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產品詳情

產品描述：

綿羊三碘甲狀腺原氨酸(T3)酶聯免疫試劑盒（CSB-EQ027510SH）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的T3含量。試劑盒檢測范圍為0.5 ng/mL-8 ng/mL，靈敏度為0.5 ng/mL。適用于科研領域中對甲狀腺激素代謝調控機制的研究；適用于動物生理學研究中甲狀腺功能評估、內分泌干擾物效應分析、藥物干預實驗中的激素水平監測，或體外細胞模型中甲狀腺激素分泌機制研究；為探究甲狀腺相關代謝通路、疾病模型構建及藥理學研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

T3
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.5 ng/mL-8 ng/mL
靈敏度：

0.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheepT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	90-100
1:2	Average %	87
	Range %	85-96
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	82-99

回收率：

The recovery of sheep T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	85-102
EDTA plasma (n=4)	96	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.