- A 96-well Assay plate --The 96-well plate has been pre-coated with an anti-human Ts antibody.
- Standard(10 x concentrate) (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- Biotin-conjugated T3 (1 x 6 ml)
- HRP-avidin (1 x 6 ml) --Catalyze the TMB, eliciting a chromogenic reaction.
- Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances.
- Substrate A (1x 7 ml) --Mix with Substrate B.
- Substrate B (1x 7 ml) --Mix with Substrate A.
- Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
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