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中文名稱：大鼠載脂蛋白A1(Apo-A1)酶聯免疫試劑盒
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貨號：CSB-E08105r
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規格：96T/48T
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價格：￥3900/￥2500
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其他：
產品詳情
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產品描述：大鼠載脂蛋白A1(Apo-A1)酶聯免疫試劑盒（CSB-E08105r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOA1含量。APOA1是重要靶點。它是高密度脂蛋白（HDL）的主要載脂蛋白，與膽固醇逆轉運相關，有助于將外周組織膽固醇轉運回肝臟代謝。研究發現其在動脈粥樣硬化等疾病進程中發揮關鍵作用，通過提高其水平或活性有望改善脂質代謝、防治相關疾病。試劑盒檢測范圍為0.14 ng/ml-100 ng/ml.，靈敏度為0.14 ng/ml.。本試劑盒適用于基礎科研中對大鼠模型脂代謝機制的研究，例如通過檢測不同實驗條件下（如基因干預、飲食調控或藥物處理）血清或組織中的Apo - A1濃度變化，評估心血管疾病模型的病理進程或干預效果。為探索Apo - A1在脂質穩態及代謝性疾病中的功能提供高效研究工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Apoa1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I)] ELISA kit
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縮寫：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：0.14 ng/ml-100 ng/ml.
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靈敏度：0.14 ng/ml.
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Cardiovascular
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1000
Average %
94
Range %
88-108
1:2000
Average %
97
Range %
91-105
1:4000
Average %
103
Range %
98-108
1:8000
Average %
93
Range %
86-98
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回收率：
The recovery of rat Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
89-102
EDTA plasma (n=4)
89
82-96
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標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
100
2.340
2.434
2.387
2.275
33.33
1.892
1.867
1.880
1.768
11.11
1.157
1.128
1.142
1.031
3.70
0.625
0.639
0.632
0.521
1.23
0.297
0.311
0.304
0.192
0.41
0.188
0.193
0.190
0.079
0.14
0.134
0.140
0.137
0.025
0
0.108
0.115
0.112
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數據處理：
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貨期：3-5 working days
引用文獻
- Hypolipidemic and anti-inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model Chernukha IM.et al,Anim Sci J,2018
- Effect of n-3 PUFA supplementation at different EPA: DHA ratios on the spontaneously hypertensive obese rat model of the metabolic syndrome Molinar-Toribio E et al,Br J Nutr,2015
產品評價
相關產品
相關問答
My samples are hemolyzed....could it be any problem with this samples when using the kits?
If it is slightly hemolyzed, the impact is small and the samples can be used.
The sensitivity of this kit is relatively good, but we still advice you to use dual-wavelength detection to eliminate the impact.
靶點詳情
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最新研究進展：APOA1是一種高密度脂蛋白(HDL)的主要成分，參與調節膽固醇代謝和清除血管內的膽固醇。最新研究表明，APOA1可以抑制乳腺癌的惡性轉化和肺癌細胞的增殖，可能成為腫瘤治療的新靶點。此外，APOA1水平與糖尿病、心血管疾病和肝病等多種疾病相關。
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功能：Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
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基因功能參考文獻：
- both the route of administration and the formulation of apoA-I peptide significantly affect its pharmacokinetics and pharmacodynamics. PMID: 27881716
- ABCA1 is diminished and ABCG1 is increased by N-acetylcysteine, which counteracts the reduction in apo A-I-mediated cholesterol efflux PMID: 25305669
- Apolipoprotein A-I induces tubulin phosphorylation in association with cholesterol release in fetal rat astrocytes. PMID: 24814947
- ApoA-I may be a protective blood-borne factor involved in the remote ischemic preconditioning mechanism. PMID: 24155931
- It maintains cholesterol homeostasis. PMID: 24117066
- on neonatal rat ventricular cardiomyocytes, the aldosterone-dependent chronotropic effects of antiapoA-1 IgG involve the nongenomic activation of L-type calcium channels PMID: 22253414
- translocation of cholesterol and phospholipid into the cytosol is related to the apo A-I-mediated HDL assembly in astrocytes through functional association with caveolin-1 and a cyclosporin A-sensitive cyclophilin protein(s). PMID: 11773045
- ABCA1-dependent cholesterol mobilization to apoA-I increased new cholesterol synthesis, indicating depletion of the regulatory pool of hepatocyte cholesterol during HDL formation PMID: 14993246
- The association of CLPP with microtubules is mediated by caveolin-1, induced by apoA-I and regulates intracellular cholesterol trafficking for assembly of cellular lipids to apoA-I-HDL. PMID: 16606364
- Data show that a complex of apolipoprotein A-I and pregnenolone modulate the rate of protein biosynthesis in liver cells. PMID: 18457017
- ApoA-I reduced I/R-induced inflammatory responses, decreased renal microscopic damage and improved renal function. ApoA-I protects kidney from I/R injury by inhibiting inflammatory cytokines release and neutrophil infiltration and activation. PMID: 18535924
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亞細胞定位：Secreted.
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蛋白家族：Apolipoprotein A1/A4/E family
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組織特異性：Major protein of plasma HDL, also found in chylomicrons.
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