Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

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中文名稱：

大鼠NAD依賴性蛋白脫乙酰酶sirtuin-1(SIRT1)酶聯(lián)免疫試劑盒
貨號：

CSB-EL021339RA
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠NAD依賴性蛋白脫乙酰酶sirtuin-1(SIRT1)酶聯(lián)免疫試劑盒（CSB-EL021339RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SIRT1含量。SIRT1 是一種具有重要生物學(xué)意義的靶點。它參與細(xì)胞代謝、衰老、應(yīng)激反應(yīng)等多種生理過程。研究發(fā)現(xiàn)它通過去乙酰化作用調(diào)控下游蛋白功能，在癌癥、代謝疾病等方面有潛在機(jī)制，是當(dāng)前藥物研發(fā)和基礎(chǔ)研究關(guān)注的重要靶點。試劑盒檢測范圍為34.3 pg/mL-2200 pg/mL，適用于體外研究，如通過檢測不同處理條件下（如藥物干預(yù)、基因修飾）的SIRT1表達(dá)變化，探索其在能量穩(wěn)態(tài)調(diào)節(jié)、神經(jīng)保護(hù)或抗氧化通路中的作用機(jī)制，也可用于肥胖、糖尿病等代謝模型動物的生物標(biāo)志物分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

RP11-57G10.3, SIR2L1, SIR2alpha|sir2-like 1|sirtuin 1|sirtuin type 1
縮寫：

SIRT1
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

34.3 pg/mL-2200 pg/mL
靈敏度：

8.5 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SIRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-94
1:2	Average %	102
	Range %	97-105
1:4	Average %	91
	Range %	87-95
1:8	Average %	98
	Range %	92-102

回收率：

The recovery of rat SIRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	99	95-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2200	2.357	2.272	2.315	2.209
1100	1.976	1.873	1.925	1.819
550	1.582	1.486	1.534	1.428
275	1.141	1.158	1.150	1.044
137.5	0.761	0.757	0.759	0.653
68.75	0.436	0.425	0.431	0.325
34.3	0.262	0.266	0.264	0.158
0	0.107	0.105	0.106

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Various endurance training intensities improve GFR and Up-regulate AQP2/GSK3β in lithium-induced nephropathic rats S Saberi, MA Rajizadeh, M Khaksari, A Saber,BMC nephrology,2025
Cyproheptadine causes apoptosis and decreases inflammation by disrupting thiol/disulfide balance and enhancing the levels of SIRT1 in C6 glioblastoma cells Sedat Kacar,Toxicology In Vitro,2021
Specific components of child gestures at 14 months are associated with preschoolers' language skills Y Ibara,J-STAGE,2020
Potential renoprotective effects of piceatannol in ameliorating the early-stage nephropathy associated with obesity in obese Zucker rats. Llarena M.et al,J Physiol Biochem.,2015

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.