Rat Mucin-5 subtype AC(MUC5AC)ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠粘蛋白/粘液素5AC(MUC5AC)酶聯(lián)免疫試劑盒（CSB-E16223r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MUC5AC含量。MUC5AC 是一種黏蛋白。它在呼吸道等部位發(fā)揮重要作用，其異常表達與哮喘、慢性阻塞性肺疾病等多種呼吸系統(tǒng)疾病密切相關。研究機制方面，主要聚焦于調(diào)控其表達、分泌的信號通路，以探索通過干預這些通路來治療相關疾病的方法。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，本試劑盒適用于基礎科研領域，例如研究呼吸道疾病模型（如哮喘、慢性阻塞性肺病）中黏液分泌調(diào)控機制，評估胃腸道黏膜屏障功能變化，或探索藥物、環(huán)境因素對黏液代謝通路的影響，為相關生物標志物研究及分子機制解析提供可靠工具。適用于實驗室常規(guī)檢測及高通量篩查需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

MUC5AC
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	94
	Range %	87-97
1:10	Average %	97
	Range %	92-102
1:20	Average %	92
	Range %	88-97
1:40	Average %	95
	Range %	89-100

回收率：

The recovery of rat MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-95
EDTA plasma (n=4)	99	95-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.